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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB8049 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects equine IL-1 beta in ELISAs.
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutral-ization by Equine IL-1 beta/IL-1F2 Antibody. Recombinant Equine IL-1 beta/IL-1F2 induces proliferation in the D10.G4.1 mouse helper T cell line in the presence of concanavalin A (1.25 μg/mL) in a dose-depend-ent manner (orange line), as measured by the Resazurin (Catalog # AR002). Under these conditions, proliferation elicited by IL-1 beta/IL-1F2 is neutralized (green line) by increasing concen-trations of Mouse Anti-Equine IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB8049). The ND50 is typically 2-10 μg/mL.
