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- Antigen structure
- References [21]
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- Validations
- Western blot [1]
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- Blocking/Neutralizing [1]
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- Product number
- MAB201-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 beta/IL-1F2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-1 beta/IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta and rpIL-1 beta and no cross-reactivity with rrIL-1 beta , rmIL-1 alpha , rhIL-1ra, rmIL-1ra, or rrIL-1 alpha .
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P01584- Isotype
- IgG
- Antibody clone number
- 8516
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Developmental induction of human T-cell responses against Candida albicans and Aspergillus fumigatus.
Extracellular Neutrophil Proteases Are Efficient Regulators of IL-1, IL-33, and IL-36 Cytokine Activity but Poor Effectors of Microbial Killing.
The Vasoreparative Function of Myeloid Angiogenic Cells Is Impaired in Diabetes Through the Induction of IL1β.
Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific.
Adipose Progenitor Cell Secretion of GM-CSF and MMP9 Promotes a Stromal and Immunological Microenvironment That Supports Breast Cancer Progression.
Downregulation of estrogen receptor and modulation of growth of breast cancer cell lines mediated by paracrine stromal cell signals.
Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses.
NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis.
Differential IL-1β secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability.
Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue.
Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-β Responses.
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
IL-26 is overexpressed in rheumatoid arthritis and induces proinflammatory cytokine production and Th17 cell generation.
Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β.
TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity.
Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway.
Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum.
Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia.
Vogel K, Pierau M, Arra A, Lampe K, Schlueter D, Arens C, Brunner-Weinzierl MC
Scientific reports 2018 Nov 15;8(1):16904
Scientific reports 2018 Nov 15;8(1):16904
Extracellular Neutrophil Proteases Are Efficient Regulators of IL-1, IL-33, and IL-36 Cytokine Activity but Poor Effectors of Microbial Killing.
Clancy DM, Sullivan GP, Moran HBT, Henry CM, Reeves EP, McElvaney NG, Lavelle EC, Martin SJ
Cell reports 2018 Mar 13;22(11):2937-2950
Cell reports 2018 Mar 13;22(11):2937-2950
The Vasoreparative Function of Myeloid Angiogenic Cells Is Impaired in Diabetes Through the Induction of IL1β.
Chambers SEJ, O'Neill CL, Guduric-Fuchs J, McLoughlin KJ, Liew A, Egan AM, O'Brien T, Stitt AW, Medina RJ
Stem cells (Dayton, Ohio) 2018 Jun;36(6):834-843
Stem cells (Dayton, Ohio) 2018 Jun;36(6):834-843
Human Scavenger Receptor A1-Mediated Inflammatory Response to Silica Particle Exposure Is Size Specific.
Nishijima N, Hirai T, Misato K, Aoyama M, Kuroda E, Ishii KJ, Higashisaka K, Yoshioka Y, Tsutsumi Y
Frontiers in immunology 2017;8:379
Frontiers in immunology 2017;8:379
Adipose Progenitor Cell Secretion of GM-CSF and MMP9 Promotes a Stromal and Immunological Microenvironment That Supports Breast Cancer Progression.
Reggiani F, Labanca V, Mancuso P, Rabascio C, Talarico G, Orecchioni S, Manconi A, Bertolini F
Cancer research 2017 Sep 15;77(18):5169-5182
Cancer research 2017 Sep 15;77(18):5169-5182
Downregulation of estrogen receptor and modulation of growth of breast cancer cell lines mediated by paracrine stromal cell signals.
Huang J, Woods P, Normolle D, Goff JP, Benos PV, Stehle CJ, Steinman RA
Breast cancer research and treatment 2017 Jan;161(2):229-243
Breast cancer research and treatment 2017 Jan;161(2):229-243
Periodontitis-associated pathogens P. gingivalis and A. actinomycetemcomitans activate human CD14(+) monocytes leading to enhanced Th17/IL-17 responses.
Cheng WC, van Asten SD, Burns LA, Evans HG, Walter GJ, Hashim A, Hughes FJ, Taams LS
European journal of immunology 2016 Sep;46(9):2211-21
European journal of immunology 2016 Sep;46(9):2211-21
NLRP3 inflammasome: a novel link between lipoproteins and atherosclerosis.
Li WL, Hua LG, Qu P, Yan WH, Ming C, Jun YD, Yuan LD, Nan N
Archives of medical science : AMS 2016 Oct 1;12(5):950-958
Archives of medical science : AMS 2016 Oct 1;12(5):950-958
Differential IL-1β secretion by monocyte subsets is regulated by Hsp27 through modulating mRNA stability.
Hadadi E, Zhang B, Baidžajevas K, Yusof N, Puan KJ, Ong SM, Yeap WH, Rotzschke O, Kiss-Toth E, Wilson H, Wong SC
Scientific reports 2016 Dec 15;6:39035
Scientific reports 2016 Dec 15;6:39035
Development of a Novel Model for the Assessment of Dead-Space Management in Soft Tissue.
Oliver RA, Lovric V, Yu Y, Christou C, Aiken SS, Cooper JJ, Walsh WR
PloS one 2015;10(8):e0136514
PloS one 2015;10(8):e0136514
Herpesvirus Genome Recognition Induced Acetylation of Nuclear IFI16 Is Essential for Its Cytoplasmic Translocation, Inflammasome and IFN-β Responses.
Ansari MA, Dutta S, Veettil MV, Dutta D, Iqbal J, Kumar B, Roy A, Chikoti L, Singh VV, Chandran B
PLoS pathogens 2015 Jul;11(7):e1005019
PLoS pathogens 2015 Jul;11(7):e1005019
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
Nicolaidou V, Wong MM, Redpath AN, Ersek A, Baban DF, Williams LM, Cope AP, Horwood NJ
PloS one 2012;7(7):e39871
PloS one 2012;7(7):e39871
IL-26 is overexpressed in rheumatoid arthritis and induces proinflammatory cytokine production and Th17 cell generation.
Corvaisier M, Delneste Y, Jeanvoine H, Preisser L, Blanchard S, Garo E, Hoppe E, Barré B, Audran M, Bouvard B, Saint-André JP, Jeannin P
PLoS biology 2012;10(9):e1001395
PLoS biology 2012;10(9):e1001395
Pathogen-induced human TH17 cells produce IFN-γ or IL-10 and are regulated by IL-1β.
Zielinski CE, Mele F, Aschenbrenner D, Jarrossay D, Ronchi F, Gattorno M, Monticelli S, Lanzavecchia A, Sallusto F
Nature 2012 Apr 26;484(7395):514-8
Nature 2012 Apr 26;484(7395):514-8
TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
Aliahmadi E, Gramlich R, Grützkau A, Hitzler M, Krüger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
European journal of immunology 2009 May;39(5):1221-30
European journal of immunology 2009 May;39(5):1221-30
Flightless-I regulates proinflammatory caspases by selectively modulating intracellular localization and caspase activity.
Li J, Yin HL, Yuan J
The Journal of cell biology 2008 Apr 21;181(2):321-33
The Journal of cell biology 2008 Apr 21;181(2):321-33
Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells.
Acosta-Rodriguez EV, Napolitani G, Lanzavecchia A, Sallusto F
Nature immunology 2007 Sep;8(9):942-9
Nature immunology 2007 Sep;8(9):942-9
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Digestive diseases and sciences 2007 Oct;52(10):2775-83
Digestive diseases and sciences 2007 Oct;52(10):2775-83
Neutrophil-derived elastase induces TGF-beta1 secretion in human airway smooth muscle via NF-kappaB pathway.
Lee KY, Ho SC, Lin HC, Lin SM, Liu CY, Huang CD, Wang CH, Chung KF, Kuo HP
American journal of respiratory cell and molecular biology 2006 Oct;35(4):407-14
American journal of respiratory cell and molecular biology 2006 Oct;35(4):407-14
Intravenous human interleukin-1alpha impairs memory processing in mice: dependence on blood-brain barrier transport into posterior division of the septum.
Banks WA, Farr SA, La Scola ME, Morley JE
The Journal of pharmacology and experimental therapeutics 2001 Nov;299(2):536-41
The Journal of pharmacology and experimental therapeutics 2001 Nov;299(2):536-41
Amyloid beta and amylin fibrils induce increases in proinflammatory cytokine and chemokine production by THP-1 cells and murine microglia.
Yates SL, Burgess LH, Kocsis-Angle J, Antal JM, Dority MD, Embury PB, Piotrkowski AM, Brunden KR
Journal of neurochemistry 2000 Mar;74(3):1017-25
Journal of neurochemistry 2000 Mar;74(3):1017-25
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-1 beta/IL-1F2 by Western Blot. Western blot shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 200 nM PMA for 24 hours and 10 µg/mL LPS and 3 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB201) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-1 beta/IL-1F2 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 beta/IL-1F2 in Human PBMCs. IL-1 beta/IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1 beta/IL-1F2 in Human PBMCs. IL-1 beta/IL-1F2 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB201) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-1 beta/IL-1F2 and Neutralization by Human IL-1 beta/IL-1F2 Antibody. Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) stimulates proliferation in the the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-1 beta/IL-1F2 (50 pg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 beta/IL-1F2 Monoclonal Antibody (Catalog # MAB201). The ND50 is typically 0.001-0.003 µg/mL in the presence of concanavalin A (1.25 µg/mL).
