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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC4013F - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-1 beta/IL-1F2 Fluorescein-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IL-1 beta/IL-1F2 in direct ELISAs. In direct ELISAs, approximately 75% cross-reactivity with recombinant guinea pig IL-1 beta , 5-15% cross-reactivitiy with recombinant canine, cotton rat, feline, equine, and rat IL-1 beta , and no cross-reactivity with recombinant human, porcine, and rhesus IL-1 beta is observed. No cross-reactivity with recombinant human (rh) Pro-IL-1 beta , rhIL-1F7, rhIL-1F10, rhIL-36 gamma , recombinant mouse (rm) IL-1 alpha , rmIL-18, rmIL-36Ra, rmIL-36 alpha , or rmIL-36 beta is observed.
- Reactivity
- Mouse
- Host
- Rat
- Antigen sequence
P10749- Isotype
- IgG
- Antibody clone number
- 166931
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-1 beta/IL-1F2 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes, untreated (open histogram), or treated with LPS for 48 hours (filled histogram) were stained with Rat Anti-Mouse IL-1 beta/IL-1F2 Fluorescein-conjugated Monoclonal Antibody (Catalog # IC4013F, filled histogram) or isotype control antibody (Catalog # IC013F, data not shown). Histograms were gated on blasting splenocytes. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
