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- Antibody Data
- Antigen structure
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- ELISA [1]
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- Product number
- 511704 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#511704, RRID:AB_1236449
- Product name
- Biotin anti-human IL-1
- Antibody type
- Monoclonal
- Antigen
- IL-1b
- Description
- IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 500 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4°C. Do not freeze.
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- Human PBMCs, stimulated with 1 μg/ml of LPS for 8 h and treated with Brefeldin A during the last 4 h, were prepared by cytospin, fixed and permeabilized on a slide and then treated with endogenous biotin blocking kit (Vector labs). Slides were stained with anti-human IL-1β biotin (clone H1b-98) and Dylight™ 649 streptavidin (green) and counterstained with DAPI (blue). Images were acquired with an Olympus FV10i confocal microscope. Images courtesy of Teresa Rodriguez, Darius Schneider, and Matthias von Herrath, LaJolla Institute for Allergy and Immunology.
- Conjugate
- Biotin
