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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- IC201P - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1 beta/IL-1F2 PE-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-1 beta/IL-1F2 in Western blots. Shows less than 5% cross-reactivity with recombinant mouse (rm) IL-1 beta and recombinant porcine IL-1 beta and no cross-reactivity with recombinant rat (rr) IL-1 beta , rmIL-1 alpha , recombinant human IL-1ra, rmIL-1ra, or rrIL-1 alpha .
- Reactivity
- Human
- Host
- Mouse
- Antigen sequence
P01584- Isotype
- IgG
- Antibody clone number
- 8516
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Identification of IDO-positive and IDO-negative human dendritic cells after activation by various proinflammatory stimuli.
A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.
A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC).
Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production.
Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production.
Von Bubnoff D, Scheler M, Wilms H, Fimmers R, Bieber T
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):6701-9
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):6701-9
A novel small molecule inhibitor of signal transducers and activators of transcription 3 reverses immune tolerance in malignant glioma patients.
Hussain SF, Kong LY, Jordan J, Conrad C, Madden T, Fokt I, Priebe W, Heimberger AB
Cancer research 2007 Oct 15;67(20):9630-6
Cancer research 2007 Oct 15;67(20):9630-6
A reducing microenvironment leads to the generation of FcepsilonRIhigh inflammatory dendritic epidermal cells (IDEC).
Novak N, Kraft S, Haberstok J, Geiger E, Allam P, Bieber T
The Journal of investigative dermatology 2002 Oct;119(4):842-9
The Journal of investigative dermatology 2002 Oct;119(4):842-9
Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production.
Wang L, Das H, Kamath A, Li L, Bukowski JF
Journal of immunology (Baltimore, Md. : 1950) 2002 May 15;168(10):4889-96
Journal of immunology (Baltimore, Md. : 1950) 2002 May 15;168(10):4889-96
Human V gamma 2V delta 2 T cells augment migration-inhibitory factor secretion and counteract the inhibitory effect of glucocorticoids on IL-1 beta and TNF-alpha production.
Wang L, Das H, Kamath A, Li L, Bukowski JF
Journal of immunology (Baltimore, Md. : 1950) 2002 May 15;168(10):4889-96
Journal of immunology (Baltimore, Md. : 1950) 2002 May 15;168(10):4889-96
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of IL-1 beta/IL-1F2 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes stimulated with LPS were stained with Mouse Anti-Human CD14 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB3832F) and either (A) Mouse Anti-Human IL-1 beta/IL-1F2 PE-conjugated Monoclonal Antibody (Catalog # IC201P) or (B) Mouse IgG1 Phycoerythrin Isotype Control (Catalog # IC002P). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
