MAB936-100

antibody from R&D Systems

Targeting: MMP9 CLG4B

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB936-100

Provider

R&D Systems

Product name

Human/Primate MMP-9 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human MMP-9 in Western blots. Does not react with the C-terminal truncated form (65 kDa) of recombinant human (rh) MMP-9. In Western blots, approximately 5% cross-reactivity with rhMMP-2 and no cross-reactivity with rhMMP-1 or rhMMP-3 is observed.

Reactivity

Human, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

36020

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Low Plasma IL-8 Levels During Chemotherapy Are Predictive of Excellent Long-Term Survival in Metastatic Breast Cancer.

Tiainen L, Hämäläinen M, Luukkaala T, Tanner M, Lahdenperä O, Vihinen P, Jukkola A, Karihtala P, Moilanen E, Kellokumpu-Lehtinen PL
Clinical breast cancer 2019 Aug;19(4):e522-e533

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Neutrophils from vasculitis patients exhibit an increased propensity for activation by anti-neutrophil cytoplasmic antibodies.

Ohlsson SM, Ohlsson S, Söderberg D, Gunnarsson L, Pettersson Å, Segelmark M, Hellmark T
Clinical and experimental immunology 2014 Jun;176(3):363-72

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In vitro secretion of matrix metalloprotease 9 is a prognostic marker in childhood acute lymphoblastic leukemia.

Schneider P, Costa O, Legrand E, Bigot D, Lecleire S, Grassi V, Vannier JP, Vasse M
Leukemia research 2010 Jan;34(1):24-31

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Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.

Zhao Z, McCloud B, Fleming R, Klempner MS
Biochemical and biophysical research communications 2007 Jun 29;358(2):528-33

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Distinct synovial immunopathologic characteristics of juvenile-onset spondylarthritis and other forms of juvenile idiopathic arthritis.

Kruithof E, Van den Bossche V, De Rycke L, Vandooren B, Joos R, Cañete JD, Tak PP, Boots AM, Veys EM, Baeten D
Arthritis and rheumatism 2006 Aug;54(8):2594-604

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Neutrophil-derived metalloproteinase-9 predicts healing quality after sinus surgery.

Watelet JB, Demetter P, Claeys C, Van Cauwenberge P, Cuvelier C, Bachert C
The Laryngoscope 2005 Jan;115(1):56-61

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

MMP-9 in SW1353 Human Cell Line. MMP-9 was detected in immersion fixed SW1353 human chondrosarcoma cell line using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

MMP-9 in Human Lung. MMP-9 was detected in immersion fixed paraffin-embedded sections of human lung using Mouse Anti-Human/Primate MMP-9 Monoclonal Antibody (Catalog # MAB936) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in macrophages. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.