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- Antibody Data
- Antigen structure
- References [52]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AF909 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse MMP-9 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse MMP-9 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human (rh) MMP-9 is observed and less than 2% cross-reactivity with rhMMP-1, -2, -3, -7, -8, -10, -12, and -13 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P41245- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Platelets isolated from an Alzheimer mouse damage healthy cortical vessels and cause inflammation in an organotypic ex vivo brain slice model.
MicroRNA-21 Knockout Exacerbates Angiotensin II-Induced Thoracic Aortic Aneurysm and Dissection in Mice With Abnormal Transforming Growth Factor-β-SMAD3 Signaling.
Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells.
Endotoxemia shifts neutrophils with TIMP-free gelatinase B/MMP-9 from bone marrow to the periphery and induces systematic upregulation of TIMP-1.
Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization Controls Endochondral Ossification through Hyaluronan Metabolism.
TIMP-1 is upregulated, but not essential in hepatic fibrogenesis and carcinogenesis in mice.
Bexarotene targets autophagy and is protective against thromboembolic stroke in aged mice with tauopathy.
Thymocyte development in the absence of matrix metalloproteinase-9/gelatinase B.
Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries.
Matrix Metalloproteinase-2 (MMP-2) Gene Deletion Enhances MMP-9 Activity, Impairs PARP-1 Degradation, and Exacerbates Hepatic Ischemia and Reperfusion Injury in Mice.
Possible dual role of decorin in abdominal aortic aneurysm.
Cytomegalovirus initiates infection selectively from high-level β1 integrin-expressing cells in the brain.
Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury.
Hepatic ischemia and reperfusion injury in the absence of myeloid cell-derived COX-2 in mice.
The ω-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
The mineral dissolution function of osteoclasts is dispensable for hypertrophic cartilage degradation during long bone development and growth.
MMP-9 deficiency shelters endothelial PECAM-1 expression and enhances regeneration of steatotic livers after ischemia and reperfusion injury.
Platelets guide the formation of early metastatic niches.
KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
Chemerin neutralization blocks hematopoietic stem cell osteoclastogenesis.
CD43-mediated IFN-γ production by CD8+ T cells promotes abdominal aortic aneurysm in mice.
Thrombospondin-1 induction in the diabetic myocardium stabilizes the cardiac matrix in addition to promoting vascular rarefaction through angiopoietin-2 upregulation.
The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration.
Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
Immunodominant fragments of myelin basic protein initiate T cell-dependent pain.
Osteopontin-stimulated expression of matrix metalloproteinase-9 causes cardiomyopathy in the mdx model of Duchenne muscular dystrophy.
Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
Matrix metalloproteinase-9 regulates tumor cell invasion through cleavage of protease nexin-1.
Tumor necrosis factor-α regulates distinct molecular pathways and gene networks in cultured skeletal muscle cells.
TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB.
Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
Molecular role of Cx37 in advanced atherosclerosis: a micro-array study.
A TLR2 agonist in German cockroach frass activates MMP-9 release and is protective against allergic inflammation in mice.
Inducible nitric oxide synthase deficiency impairs matrix metalloproteinase-9 activity and disrupts leukocyte migration in hepatic ischemia/reperfusion injury.
Matrix metalloproteinase-9 deficiency worsens lung injury in a model of bronchopulmonary dysplasia.
Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse.
Cyclooxygenase-2 deficiency enhances Th2 immune responses and impairs neutrophil recruitment in hepatic ischemia/reperfusion injury.
Cyclooxygenase-2 deficiency enhances Th2 immune responses and impairs neutrophil recruitment in hepatic ischemia/reperfusion injury.
A3 adenosine receptor signaling influences pulmonary inflammation and fibrosis.
Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
A novel method to establish microglia-free astrocyte cultures: comparison of matrix metalloproteinase expression profiles in pure cultures of astrocytes and microglia.
A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases.
Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases.
Angiostatin inhibits monocyte/macrophage migration via disruption of actin cytoskeleton.
Functional relevance of the IL-23-IL-17 axis in lungs in vivo.
Matrix metalloproteinase-2 (MMP-2) regulates astrocyte motility in connection with the actin cytoskeleton and integrins.
Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis.
Ultrastructural localization of matrix metalloproteinase-9 in eosinophils from the cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis.
Kniewallner KM, Foidl BM, Humpel C
Scientific reports 2018 Oct 19;8(1):15483
Scientific reports 2018 Oct 19;8(1):15483
MicroRNA-21 Knockout Exacerbates Angiotensin II-Induced Thoracic Aortic Aneurysm and Dissection in Mice With Abnormal Transforming Growth Factor-β-SMAD3 Signaling.
Huang X, Yue Z, Wu J, Chen J, Wang S, Wu J, Ren L, Zhang A, Deng P, Wang K, Wu C, Ding X, Ye P, Xia J
Arteriosclerosis, thrombosis, and vascular biology 2018 May;38(5):1086-1101
Arteriosclerosis, thrombosis, and vascular biology 2018 May;38(5):1086-1101
Tea Polysaccharide Prevents Colitis-Associated Carcinogenesis in Mice by Inhibiting the Proliferation and Invasion of Tumor Cells.
Liu LQ, Li HS, Nie SP, Shen MY, Hu JL, Xie MY
International journal of molecular sciences 2018 Feb 8;19(2)
International journal of molecular sciences 2018 Feb 8;19(2)
Endotoxemia shifts neutrophils with TIMP-free gelatinase B/MMP-9 from bone marrow to the periphery and induces systematic upregulation of TIMP-1.
Vandooren J, Swinnen W, Ugarte-Berzal E, Boon L, Dorst D, Martens E, Opdenakker G
Haematologica 2017 Oct;102(10):1671-1682
Haematologica 2017 Oct;102(10):1671-1682
Hyaluronan-Binding Protein Involved in Hyaluronan Depolymerization Controls Endochondral Ossification through Hyaluronan Metabolism.
Shimoda M, Yoshida H, Mizuno S, Hirozane T, Horiuchi K, Yoshino Y, Hara H, Kanai Y, Inoue S, Ishijima M, Okada Y
The American journal of pathology 2017 May;187(5):1162-1176
The American journal of pathology 2017 May;187(5):1162-1176
TIMP-1 is upregulated, but not essential in hepatic fibrogenesis and carcinogenesis in mice.
Thiele ND, Wirth JW, Steins D, Koop AC, Ittrich H, Lohse AW, Kluwe J
Scientific reports 2017 Apr 6;7(1):714
Scientific reports 2017 Apr 6;7(1):714
Bexarotene targets autophagy and is protective against thromboembolic stroke in aged mice with tauopathy.
Huuskonen MT, Loppi S, Dhungana H, Keksa-Goldsteine V, Lemarchant S, Korhonen P, Wojciechowski S, Pollari E, Valonen P, Koponen J, Takashima A, Landreth G, Goldsteins G, Malm T, Koistinaho J, Kanninen KM
Scientific reports 2016 Sep 14;6:33176
Scientific reports 2016 Sep 14;6:33176
Thymocyte development in the absence of matrix metalloproteinase-9/gelatinase B.
Gounko NV, Martens E, Opdenakker G, Rybakin V
Scientific reports 2016 Jul 19;6:29852
Scientific reports 2016 Jul 19;6:29852
Smooth muscle cell-specific Tgfbr1 deficiency attenuates neointimal hyperplasia but promotes an undesired vascular phenotype for injured arteries.
Liao M, Yang P, Wang F, Berceli SA, Ali YH, Chan KL, Jiang Z
Physiological reports 2016 Dec;4(23)
Physiological reports 2016 Dec;4(23)
Matrix Metalloproteinase-2 (MMP-2) Gene Deletion Enhances MMP-9 Activity, Impairs PARP-1 Degradation, and Exacerbates Hepatic Ischemia and Reperfusion Injury in Mice.
Kato H, Duarte S, Liu D, Busuttil RW, Coito AJ
PloS one 2015;10(9):e0137642
PloS one 2015;10(9):e0137642
Possible dual role of decorin in abdominal aortic aneurysm.
Ueda K, Yoshimura K, Yamashita O, Harada T, Morikage N, Hamano K
PloS one 2015;10(3):e0120689
PloS one 2015;10(3):e0120689
Cytomegalovirus initiates infection selectively from high-level β1 integrin-expressing cells in the brain.
Kawasaki H, Kosugi I, Sakao-Suzuki M, Meguro S, Arai Y, Tsutsui Y, Iwashita T
The American journal of pathology 2015 May;185(5):1304-23
The American journal of pathology 2015 May;185(5):1304-23
Molecular hydrogen in drinking water protects against neurodegenerative changes induced by traumatic brain injury.
Dohi K, Kraemer BC, Erickson MA, McMillan PJ, Kovac A, Flachbartova Z, Hansen KM, Shah GN, Sheibani N, Salameh T, Banks WA
PloS one 2014;9(9):e108034
PloS one 2014;9(9):e108034
Hepatic ischemia and reperfusion injury in the absence of myeloid cell-derived COX-2 in mice.
Duarte S, Kato H, Kuriyama N, Suko K, Ishikawa TO, Busuttil RW, Herschman HR, Coito AJ
PloS one 2014;9(5):e96913
PloS one 2014;9(5):e96913
The ω-3 polyunsaturated fatty acid, eicosapentaenoic acid, attenuates abdominal aortic aneurysm development via suppression of tissue remodeling.
Wang JH, Eguchi K, Matsumoto S, Fujiu K, Komuro I, Nagai R, Manabe I
PloS one 2014;9(5):e96286
PloS one 2014;9(5):e96286
Lymphatic regulator PROX1 determines Schlemm's canal integrity and identity.
Park DY, Lee J, Park I, Choi D, Lee S, Song S, Hwang Y, Hong KY, Nakaoka Y, Makinen T, Kim P, Alitalo K, Hong YK, Koh GY
The Journal of clinical investigation 2014 Sep;124(9):3960-74
The Journal of clinical investigation 2014 Sep;124(9):3960-74
The mineral dissolution function of osteoclasts is dispensable for hypertrophic cartilage degradation during long bone development and growth.
Touaitahuata H, Cres G, de Rossi S, Vives V, Blangy A
Developmental biology 2014 Sep 1;393(1):57-70
Developmental biology 2014 Sep 1;393(1):57-70
MMP-9 deficiency shelters endothelial PECAM-1 expression and enhances regeneration of steatotic livers after ischemia and reperfusion injury.
Kato H, Kuriyama N, Duarte S, Clavien PA, Busuttil RW, Coito AJ
Journal of hepatology 2014 May;60(5):1032-9
Journal of hepatology 2014 May;60(5):1032-9
Platelets guide the formation of early metastatic niches.
Labelle M, Begum S, Hynes RO
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 29;111(30):E3053-61
Proceedings of the National Academy of Sciences of the United States of America 2014 Jul 29;111(30):E3053-61
KLF4 regulates abdominal aortic aneurysm morphology and deletion attenuates aneurysm formation.
Salmon M, Johnston WF, Woo A, Pope NH, Su G, Upchurch GR Jr, Owens GK, Ailawadi G
Circulation 2013 Sep 10;128(11 Suppl 1):S163-74
Circulation 2013 Sep 10;128(11 Suppl 1):S163-74
Chemerin neutralization blocks hematopoietic stem cell osteoclastogenesis.
Muruganandan S, Dranse HJ, Rourke JL, McMullen NM, Sinal CJ
Stem cells (Dayton, Ohio) 2013 Oct;31(10):2172-82
Stem cells (Dayton, Ohio) 2013 Oct;31(10):2172-82
CD43-mediated IFN-γ production by CD8+ T cells promotes abdominal aortic aneurysm in mice.
Zhou HF, Yan H, Cannon JL, Springer LE, Green JM, Pham CT
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5078-85
Journal of immunology (Baltimore, Md. : 1950) 2013 May 15;190(10):5078-85
Thrombospondin-1 induction in the diabetic myocardium stabilizes the cardiac matrix in addition to promoting vascular rarefaction through angiopoietin-2 upregulation.
Gonzalez-Quesada C, Cavalera M, Biernacka A, Kong P, Lee DW, Saxena A, Frunza O, Dobaczewski M, Shinde A, Frangogiannis NG
Circulation research 2013 Dec 6;113(12):1331-44
Circulation research 2013 Dec 6;113(12):1331-44
The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration.
Kim Y, Remacle AG, Chernov AV, Liu H, Shubayev I, Lai C, Dolkas J, Shiryaev SA, Golubkov VS, Mizisin AP, Strongin AY, Shubayev VI
PloS one 2012;7(3):e33664
PloS one 2012;7(3):e33664
Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
Xue M, Dervish S, Harrison LC, Fulcher G, Jackson CJ
The Journal of biological chemistry 2012 May 11;287(20):16356-64
The Journal of biological chemistry 2012 May 11;287(20):16356-64
Immunodominant fragments of myelin basic protein initiate T cell-dependent pain.
Liu H, Shiryaev SA, Chernov AV, Kim Y, Shubayev I, Remacle AG, Baranovskaya S, Golubkov VS, Strongin AY, Shubayev VI
Journal of neuroinflammation 2012 Jun 7;9:119
Journal of neuroinflammation 2012 Jun 7;9:119
Osteopontin-stimulated expression of matrix metalloproteinase-9 causes cardiomyopathy in the mdx model of Duchenne muscular dystrophy.
Dahiya S, Givvimani S, Bhatnagar S, Qipshidze N, Tyagi SC, Kumar A
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2723-31
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 1;187(5):2723-31
Tissue inhibitor of matrix metalloproteinase 1 (TIMP1) controls adipogenesis in obesity in mice and in humans.
Meissburger B, Stachorski L, Röder E, Rudofsky G, Wolfrum C
Diabetologia 2011 Jun;54(6):1468-79
Diabetologia 2011 Jun;54(6):1468-79
Matrix metalloproteinase-9 regulates tumor cell invasion through cleavage of protease nexin-1.
Xu D, McKee CM, Cao Y, Ding Y, Kessler BM, Muschel RJ
Cancer research 2010 Sep 1;70(17):6988-98
Cancer research 2010 Sep 1;70(17):6988-98
Tumor necrosis factor-α regulates distinct molecular pathways and gene networks in cultured skeletal muscle cells.
Bhatnagar S, Panguluri SK, Gupta SK, Dahiya S, Lundy RF, Kumar A
PloS one 2010 Oct 12;5(10):e13262
PloS one 2010 Oct 12;5(10):e13262
TRE17/USP6 oncogene translocated in aneurysmal bone cyst induces matrix metalloproteinase production via activation of NF-kappaB.
Ye Y, Pringle LM, Lau AW, Riquelme DN, Wang H, Jiang T, Lev D, Welman A, Blobel GA, Oliveira AM, Chou MM
Oncogene 2010 Jun 24;29(25):3619-29
Oncogene 2010 Jun 24;29(25):3619-29
Adenosine and osteopontin contribute to the development of chronic obstructive pulmonary disease.
Schneider DJ, Lindsay JC, Zhou Y, Molina JG, Blackburn MR
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jan;24(1):70-80
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2010 Jan;24(1):70-80
Molecular role of Cx37 in advanced atherosclerosis: a micro-array study.
Derouette JP, Wong C, Burnier L, Morel S, Sutter E, Galan K, Brisset AC, Roth I, Chadjichristos CE, Kwak BR
Atherosclerosis 2009 Sep;206(1):69-76
Atherosclerosis 2009 Sep;206(1):69-76
A TLR2 agonist in German cockroach frass activates MMP-9 release and is protective against allergic inflammation in mice.
Page K, Ledford JR, Zhou P, Wills-Karp M
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 1;183(5):3400-8
Journal of immunology (Baltimore, Md. : 1950) 2009 Sep 1;183(5):3400-8
Inducible nitric oxide synthase deficiency impairs matrix metalloproteinase-9 activity and disrupts leukocyte migration in hepatic ischemia/reperfusion injury.
Hamada T, Duarte S, Tsuchihashi S, Busuttil RW, Coito AJ
The American journal of pathology 2009 Jun;174(6):2265-77
The American journal of pathology 2009 Jun;174(6):2265-77
Matrix metalloproteinase-9 deficiency worsens lung injury in a model of bronchopulmonary dysplasia.
Lukkarinen H, Hogmalm A, Lappalainen U, Bry K
American journal of respiratory cell and molecular biology 2009 Jul;41(1):59-68
American journal of respiratory cell and molecular biology 2009 Jul;41(1):59-68
Genetic deficiency of heme oxygenase-1 impairs functionality and form of an arteriovenous fistula in the mouse.
Juncos JP, Tracz MJ, Croatt AJ, Grande JP, Ackerman AW, Katusic ZS, Nath KA
Kidney international 2008 Jul;74(1):47-51
Kidney international 2008 Jul;74(1):47-51
Cyclooxygenase-2 deficiency enhances Th2 immune responses and impairs neutrophil recruitment in hepatic ischemia/reperfusion injury.
Hamada T, Tsuchihashi S, Avanesyan A, Duarte S, Moore C, Busuttil RW, Coito AJ
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1843-53
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1843-53
Cyclooxygenase-2 deficiency enhances Th2 immune responses and impairs neutrophil recruitment in hepatic ischemia/reperfusion injury.
Hamada T, Tsuchihashi S, Avanesyan A, Duarte S, Moore C, Busuttil RW, Coito AJ
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1843-53
Journal of immunology (Baltimore, Md. : 1950) 2008 Feb 1;180(3):1843-53
A3 adenosine receptor signaling influences pulmonary inflammation and fibrosis.
Morschl E, Molina JG, Volmer JB, Mohsenin A, Pero RS, Hong JS, Kheradmand F, Lee JJ, Blackburn MR
American journal of respiratory cell and molecular biology 2008 Dec;39(6):697-705
American journal of respiratory cell and molecular biology 2008 Dec;39(6):697-705
Increased gelatinase B/matrix metalloproteinase 9 (MMP-9) activity in a murine model of acute coxsackievirus B4-induced pancreatitis.
De Palma AM, Verbeken E, Van Aelst I, Van den Steen PE, Opdenakker G, Neyts J
Virology 2008 Dec 5;382(1):20-7
Virology 2008 Dec 5;382(1):20-7
A novel method to establish microglia-free astrocyte cultures: comparison of matrix metalloproteinase expression profiles in pure cultures of astrocytes and microglia.
Crocker SJ, Frausto RF, Whitton JL, Milner R
Glia 2008 Aug 15;56(11):1187-98
Glia 2008 Aug 15;56(11):1187-98
A GMCSF and IL-15 fusokine leads to paradoxical immunosuppression in vivo via asymmetrical JAK/STAT signaling through the IL-15 receptor complex.
Rafei M, Wu JH, Annabi B, Lejeune L, François M, Galipeau J
Blood 2007 Mar 1;109(5):2234-42
Blood 2007 Mar 1;109(5):2234-42
Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases.
Zozulya AL, Reinke E, Baiu DC, Karman J, Sandor M, Fabry Z
Journal of immunology (Baltimore, Md. : 1950) 2007 Jan 1;178(1):520-9
Journal of immunology (Baltimore, Md. : 1950) 2007 Jan 1;178(1):520-9
Dendritic cell transmigration through brain microvessel endothelium is regulated by MIP-1alpha chemokine and matrix metalloproteinases.
Zozulya AL, Reinke E, Baiu DC, Karman J, Sandor M, Fabry Z
Journal of immunology (Baltimore, Md. : 1950) 2007 Jan 1;178(1):520-9
Journal of immunology (Baltimore, Md. : 1950) 2007 Jan 1;178(1):520-9
Angiostatin inhibits monocyte/macrophage migration via disruption of actin cytoskeleton.
Perri SR, Annabi B, Galipeau J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Dec;21(14):3928-36
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2007 Dec;21(14):3928-36
Functional relevance of the IL-23-IL-17 axis in lungs in vivo.
Ivanov S, Bozinovski S, Bossios A, Valadi H, Vlahos R, Malmhäll C, Sjöstrand M, Kolls JK, Anderson GP, Lindén A
American journal of respiratory cell and molecular biology 2007 Apr;36(4):442-51
American journal of respiratory cell and molecular biology 2007 Apr;36(4):442-51
Matrix metalloproteinase-2 (MMP-2) regulates astrocyte motility in connection with the actin cytoskeleton and integrins.
Ogier C, Bernard A, Chollet AM, LE Diguardher T, Hanessian S, Charton G, Khrestchatisky M, Rivera S
Glia 2006 Sep;54(4):272-84
Glia 2006 Sep;54(4):272-84
Interleukin-17 as a recruitment and survival factor for airway macrophages in allergic airway inflammation.
Sergejeva S, Ivanov S, Lötvall J, Lindén A
American journal of respiratory cell and molecular biology 2005 Sep;33(3):248-53
American journal of respiratory cell and molecular biology 2005 Sep;33(3):248-53
Expression of matrix metalloproteinases subsequent to urogenital Chlamydia muridarum infection of mice.
Ramsey KH, Sigar IM, Schripsema JH, Shaba N, Cohoon KP
Infection and immunity 2005 Oct;73(10):6962-73
Infection and immunity 2005 Oct;73(10):6962-73
Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis.
Chen KM, Lee HH, Chou HL, Liu JY, Tsai BC, Lai SC
International journal of experimental pathology 2005 Apr;86(2):81-9
International journal of experimental pathology 2005 Apr;86(2):81-9
Ultrastructural localization of matrix metalloproteinase-9 in eosinophils from the cerebrospinal fluid of mice with eosinophilic meningitis caused by Angiostrongylus cantonensis.
Tseng YK, Tu WC, Lee HH, Chen KM, Chou HL, Lai SC
Annals of tropical medicine and parasitology 2004 Dec;98(8):831-41
Annals of tropical medicine and parasitology 2004 Dec;98(8):831-41
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse MMP-9 by Western Blot. Western blot shows lysates of mouse spleen tissue and mouse lung tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MMP-9 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse MMP-9 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and mouse spleen tissue, loaded at 0.2 mg/mL. A specific band was detected for MMP-9 at approximately 155 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-9 in Mouse Splenocytes. MMP-9 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- MMP-9 in Rat Brain. MMP-9 was detected in perfusion fixed frozen sections of rat brain (cingulate cortex) using 1.7 µg/mL Goat Anti-Mouse MMP-9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF909) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
