NBP2-22530

antibody from Novus Biologicals

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

NBP2-22530

Provider

Novus Biologicals

Product name

Rat Monoclonal CD44 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse, Bovine, Goat, Rabbit, Sheep

Host

Rat

Isotype

IgG

Vial size

200 ug

Concentration

1 mg/ml

Storage

Store at -20C. Avoid freeze-thaw cycles.

References

Submitted references

Tartrate-resistant acid phosphatase (TRAP/ACP5) promotes metastasis-related properties via TGFβ2/TβR and CD44 in MDA-MB-231 breast cancer cells.

Reithmeier A, Panizza E, Krumpel M, Orre LM, Branca RMM, Lehtiö J, Ek-Rylander B, Andersson G
BMC cancer 2017 Sep 15;17(1):650

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Galectin-3 drives glycosphingolipid-dependent biogenesis of clathrin-independent carriers.

Lakshminarayan R, Wunder C, Becken U, Howes MT, Benzing C, Arumugam S, Sales S, Ariotti N, Chambon V, Lamaze C, Loew D, Shevchenko A, Gaus K, Parton RG, Johannes L
Nature cell biology 2014 Jun;16(6):595-606

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: CD44 Antibody (Hermes-1) [NBP2-22530] - of Hela cell lysates.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - Analysis of CD44 in PBMC cells (green) compared to an isotype control (blue).

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 [Hermes-1] Antibody NBP2-22530AF488 (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD44 Antibody (Hermes-1) [NBP2-22530] - A surface stain was performed on U-87 cells with CD44 (Hermes-1) Antibody NBP2-22530R (blue) and a matched isotype control (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to DyLight 550.