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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX79853 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX79853, RRID:AB_11174925
- Product name
- Vimentin antibody [VI-01] (DY547)
- Antibody type
- Monoclonal
- Host
- Mouse
- Storage
- Store in the dark at 2-8¢XC. Do not freeze. Avoid prolonged exposure to light.Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is sto
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4). Lane 1,2: immunostaining with anti-Vimentin (VI-01). Lane 3,4: immunostaining with anti-human Vimentin (VI-RE/1)
- Validation comment
- WB
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547. Nuclei are stained with DAPI (blue).
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10and its detection by antibody VI-01. IgM heavy chain (76-92 kDa) andIgM light chain (25-30 kDa) indicated. Mr of vimentin is 57kDa.Lr = lysate (reducing conditions)Lnr = lysate (non-reducing conditions)IPr = immunoprecipitate (reducing conditions)IPnr = immunoprecipitate (non-reducing conditions)
