AF6127

antibody from R&D Systems

Targeting: CD44 CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

AF6127

Provider

R&D Systems

Product name

Mouse/Rat/Porcine/Equine CD44 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects mouse and rat CD44 in direct ELISAs and Western blots. In direct ELISAs, approximately 35% cross-reactivity with recombinant human CD44 is observed.

Reactivity

Mouse, Rat, Porcine

Host

Sheep

Conjugate

Unconjugated

Antigen sequence

NP_033981


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Deficient TSC1/TSC2-complex suppression of SOX9-osteopontin-AKT signalling cascade constrains tumour growth in tuberous sclerosis complex.

Jin F, Jiang K, Ji S, Wang L, Ni Z, Huang F, Li C, Chen R, Zhang H, Hu Z, Zha X
Human molecular genetics 2017 Jan 15;26(2):407-419

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Recombinant human hyaluronidase PH20 does not stimulate an acute inflammatory response and inhibits lipopolysaccharide-induced neutrophil recruitment in the air pouch model of inflammation.

Huang Z, Zhao C, Chen Y, Cowell JA, Wei G, Kultti A, Huang L, Thompson CB, Rosengren S, Frost GI, Shepard HM
Journal of immunology (Baltimore, Md. : 1950) 2014 Jun 1;192(11):5285-95

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Mouse and Rat CD44 by Western Blot. Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line, mouse spleen tissue, mouse lymph node tissue, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). Specific bands were detected for CD44 at approximately 80 to 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Rat CD44 by Simple Western^TM. Simple Western lane view shows lysates of NR8383 rat alveolar macrophage cell line, loaded at 0.2 mg/mL. A specific band was detected for CD44 at approximately 169 kDa (as indicated) using 10 µg/mL of Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CD44 in Mouse Splenocytes. CD44 was detected in immersion fixed mouse splenocytes using Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

CD44 in Porcine Mesenchymal Stem Cells. CD44 was detected in immersion fixed porcine mesenchymal stem cells using Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD44 in Equine PBMCs by Flow Cytometry. Equine peripheral blood mononuclear cells (PBMCs) were stained with Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD44 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD44 in Porcine Mesenchymal Stem Cells by Flow Cytometry. Porcine mesenchymal stem cells were stained with Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Phycoerythrin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0126).

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of CD44 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Sheep Anti-Mouse/Rat/Porcine/Equine CD44 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6127, filled histogram) or control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127).