PA5-21419
antibody from Invitrogen Antibodies
Targeting: CD44
CD44R, CSPG8, HCELL, IN, MC56, MDU2, MDU3, MIC4, Pgp1
Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
- Other assay [6]
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Validation data
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- Product number
- PA5-21419 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD44 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A549, H1299, HeLa, A431, MDA-MB-231, MDA-MB-231, MDA-MB-231 (24 µg/mL Tunicamycin treatment for 16 hr), HeLa (12 µg/mL Tunicamycin treatment for 48 hr), HeLa (12 µg/mL Tunicamycin treatment for 48 hr and shCD44-transfected 293T).
- Concentration
- 0.5 mg/mL
Submitted references The vascular bone marrow niche influences outcome in chronic myeloid leukemia via the E-selectin - SCL/TAL1 - CD44 axis.
The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer.
Godavarthy PS, Kumar R, Herkt SC, Pereira RS, Hayduk N, Weissenberger ES, Aggoune D, Manavski Y, Lucas T, Pan KT, Voutsinas JM, Wu Q, Müller MC, Saussele S, Oellerich T, Oehler VG, Lausen J, Krause DS
Haematologica 2020 Jan;105(1):136-147
Haematologica 2020 Jan;105(1):136-147
The absence of aldehyde dehydrogenase 1 A1-positive cells in benign mammary stroma is associated with risk factors for breast cancer.
Isfoss BL, Holmqvist B, Jernström H, Alm P, Olsson H
Breast cancer (Dove Medical Press) 2016;8:117-24
Breast cancer (Dove Medical Press) 2016;8:117-24
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD44/H-CAM using Whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD44/H-CAM polyclonal antibody (Product # PA5-21419) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD44/H-CAM using A) 30 µg A549 whole cell lysate and B) 30 µg H1299 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a CD44/H-CAM polyclonal antibody (Product # PA5-21419) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CD44 was performed by separating 30 µg of untreated (–) and treated (+) MDA-MB-231 whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CD44 Polyclonal Antibody (Product # PA5-21419) at a dilution of 1:7000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CD44 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a CD44 Polyclonal Antibody (Product # PA5-21419) at a dilution of 1:7000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CD44 Polyclonal Antibody (Product # PA5-21419). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CD44/H-CAM in methanol-fixed HeLa cells using a CD44/H-CAM polyclonal antibody (Product # PA5-21419) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using CD44 antibody (Product # PA5-21419) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD44 Polyclonal Antibody detects CD44 protein at cell membrane and cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: CD44 stained by CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD44 Polyclonal Antibody detects CD44 protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. CD44 stained by CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD44 Polyclonal Antibody detects CD44 protein at membrane and cytoplasm on human endometrial carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human endometrial carcinoma. CD44 Polyclonal Antibody (Product # PA5-21419) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human pancreatic tumor, using CD44 (Product # PA5-21419) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
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