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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [10]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- AF-280-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1ra/IL-1F3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-1ra/IL-1F3 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 15% cross-reactivity with recombinant mouse IL-1ra is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P18510- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Prostaglandin E2 Indicates Therapeutic Efficacy of Mesenchymal Stem Cells in Experimental Traumatic Brain Injury.
Intranasal Administration of Interleukin-1 Receptor Antagonist in a Transient Focal Cerebral Ischemia Rat Model.
IL-1 Receptor Antagonist Chimeric Protein: Context-Specific and Inflammation-Restricted Activation.
IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra.
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies.
IL-22 increases the innate immunity of tissues.
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Kota DJ, Prabhakara KS, Toledano-Furman N, Bhattarai D, Chen Q, DiCarlo B, Smith P, Triolo F, Wenzel PL, Cox CS Jr, Olson SD
Stem cells (Dayton, Ohio) 2017 May;35(5):1416-1430
Stem cells (Dayton, Ohio) 2017 May;35(5):1416-1430
Intranasal Administration of Interleukin-1 Receptor Antagonist in a Transient Focal Cerebral Ischemia Rat Model.
Lee JH, Kam EH, Kim JM, Kim SY, Kim EJ, Cheon SY, Koo BN
Biomolecules & therapeutics 2017 Mar 1;25(2):149-157
Biomolecules & therapeutics 2017 Mar 1;25(2):149-157
IL-1 Receptor Antagonist Chimeric Protein: Context-Specific and Inflammation-Restricted Activation.
Rider P, Carmi Y, Yossef R, Guttman O, Eini H, Azam T, Dinarello CA, Lewis EC
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1705-12
Journal of immunology (Baltimore, Md. : 1950) 2015 Aug 15;195(4):1705-12
IL-4 enhances IFN-lambda1 (IL-29) production by plasmacytoid DCs via monocyte secretion of IL-1Ra.
Megjugorac NJ, Gallagher GE, Gallagher G
Blood 2010 May 27;115(21):4185-90
Blood 2010 May 27;115(21):4185-90
Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers.
van Grevenstein WM, Hofland LJ, van Rossen ME, van Koetsveld PM, Jeekel J, van Eijck CH
Digestive diseases and sciences 2007 Oct;52(10):2775-83
Digestive diseases and sciences 2007 Oct;52(10):2775-83
P2X receptor characterization and IL-1/IL-1Ra release from human endothelial cells.
Wilson HL, Varcoe RW, Stokes L, Holland KL, Francis SE, Dower SK, Surprenant A, Crossman DC
British journal of pharmacology 2007 May;151(1):115-27
British journal of pharmacology 2007 May;151(1):115-27
Immunolocalization of interleukin-1 receptors in the sarcolemma and nuclei of skeletal muscle in patients with idiopathic inflammatory myopathies.
Grundtman C, Salomonsson S, Dorph C, Bruton J, Andersson U, Lundberg IE
Arthritis and rheumatism 2007 Feb;56(2):674-87
Arthritis and rheumatism 2007 Feb;56(2):674-87
IL-22 increases the innate immunity of tissues.
Wolk K, Kunz S, Witte E, Friedrich M, Asadullah K, Sabat R
Immunity 2004 Aug;21(2):241-54
Immunity 2004 Aug;21(2):241-54
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
The Journal of allergy and clinical immunology 2002 Oct;110(4):624-33
The Journal of allergy and clinical immunology 2002 Oct;110(4):624-33
T lymphocyte-mediated changes in airway smooth muscle responsiveness are attributed to induced autocrine release and actions of IL-5 and IL-1beta.
Hakonarson H, Whelan R, Leiter J, Kim C, Chen M, Campbell D, Grunstein MM
The Journal of allergy and clinical immunology 2002 Oct;110(4):624-33
The Journal of allergy and clinical immunology 2002 Oct;110(4):624-33
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-1ra/IL-1F3 by Western Blot. Western blot shows lysates of RT-4 human bladder carcinoma cell line and A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-1ra/IL-1F3 at approximately 18-20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse IL-1ra/IL-1F3 by Simple WesternTM. Simple Western lane view shows lysates of RT-4 human bladder carcinoma cell line, A431 human epithelial carcinoma cell line, human skin cancer tissue, and mouse skin cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for IL-1ra/IL-1F3 at approximately 24-26 kDa (as indicated) using 20 µg/mL of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1ra/IL-1F3 in Human Skin. IL-1ra/IL-1F3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-1ra/IL-1F3 Inhibition of IL-1 alpha/IL-1F1-dependent Cell Proliferation and Neutralization by Human IL-1ra/IL-1F3 Antibody. Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line), as measured by Resazurin (Catalog #AR002). Inhibition of Recombinant Human IL-1 alpha/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-280-NA). The ND50 is typically
