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ELISA
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB280 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1ra/IL-1F3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-1ra/IL-1F3 in ELISAs. In ELISAs, this antibody shows less than 0.2% cross-reactivity with recombinant mouse (rm) IL-1ra and no cross-reactivity with rhIL-1 alpha , rpIL-1 alpha , rmIL-1 alpha , rrIL-1 alpha , rhIL-1 beta , rpIL-1 beta , rmIL-1 beta , rrIL-1 beta , rhIL-1 RI, or rhIL-1 RII.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P18510- Isotype
- IgG
- Antibody clone number
- 10309
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Interleukin-36 potently stimulates human M2 macrophages, Langerhans cells and keratinocytes to produce pro-inflammatory cytokines.
Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
trans fatty acids and systemic inflammation in heart failure.
Dietrich D, Martin P, Flacher V, Sun Y, Jarrossay D, Brembilla N, Mueller C, Arnett HA, Palmer G, Towne J, Gabay C
Cytokine 2016 Aug;84:88-98
Cytokine 2016 Aug;84:88-98
Suppression of TNF-α and IL-1 signaling identifies a mechanism of homeostatic regulation of macrophages by IL-27.
Kalliolias GD, Gordon RA, Ivashkiv LB
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):7047-56
Journal of immunology (Baltimore, Md. : 1950) 2010 Dec 1;185(11):7047-56
Pre-interleukin-1alpha expression reduces cell growth and increases interleukin-6 production in SaOS-2 osteosarcoma cells: Differential inhibitory effect of interleukin-1 receptor antagonist (icIL-1Ra1).
Palmer G, Trolliet S, Talabot-Ayer D, Mézin F, Magne D, Gabay C
Cytokine 2005 Jul 21;31(2):153-60
Cytokine 2005 Jul 21;31(2):153-60
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
trans fatty acids and systemic inflammation in heart failure.
Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- IL-1ra/IL-1F3 Inhibition of IL-1 alpha/IL-1F1-dependent Cell Proliferation and Neutralization by Human IL-1ra/IL-1F3 Antibody. Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 alpha/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-1ra/IL-1F3 Monoclonal Antibody (Catalog # MAB280). The ND50 is typically 10-20 µg/mL in the presence of concanavalin A (1.25 µg/mL).
