Explore
Validate
Learn
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- ELISA [1]
- Proximity ligation assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000650-M03 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000650-M03, RRID:AB_581659
- Product name
- BMP2 monoclonal antibody (M03), clone 4B12
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant BMP2.
- Antigen sequence
QAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAP
PGYHAFYCHGECPFPLADHLNSTNHAIVQTLVNSV
NSKIPKACCVPTELSAISMLYLDENEKVVLKNYQD
MVVEGCGCR- Isotype
- IgG
- Antibody clone number
- 4B12
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Antibody-mediated osseous regeneration: a novel strategy for bioengineering bone by immobilized anti-bone morphogenetic protein-2 antibodies.
Freire MO, You HK, Kook JK, Choi JH, Zadeh HH
Tissue engineering. Part A 2011 Dec;17(23-24):2911-8
Tissue engineering. Part A 2011 Dec;17(23-24):2911-8
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged BMP2 is 1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between TGFB2 and BMP2. HeLa cells were stained with anti-TGFB2 rabbit purified polyclonal 1:1200 and anti-BMP2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
