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LearnH00080279-M01
antibody from Abnova Corporation
Targeting: CDK5RAP3
C53, FLJ13660, HSF-27, IC53, LZAP, MST016, OK/SW-cl.114
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
Reference
Comment
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- Product number
- H00080279-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00080279-M01, RRID:AB_606050
- Product name
- CDK5RAP3 monoclonal antibody (M01), clone 1G4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant CDK5RAP3.
- Antigen sequence
MEDHQHVPIDIQTSKLLDWLVDRRHCSLKWQSLVL
TIREKINAAIQDMPESEEIAQLLSGSYIHYFHCLR
ILDLLKGTEASTKNIFGRYSSQRMKDWQEIIALYE
KDNTYLVELSSLLVRNVNYEIPSLKKQIAKCQQLQ
QEYSRKEEECQAGAAEMREQFYHSCKQYGITGENV
RGELLALVKDLPSQLAEIGAAAQQSLGEAIDVYQA
SVGFVCESPTEQVLPMLRFVQKRGNSTVYEWRTGT
EPSVVERPHLEELPEQVAEDAIDWGDFGVEAVSEG
TDSGISAEAAGIDWGIFPESDSKDPGGDGIDWGDD
AVALQITVLEAGTQAPEGVARGPDALTLLEYTETR
NQFLDELMELEIFLAQRAVELSEEADVLSVSQFQL
APAILQGQTKEKMVTMVSVLEDLIGKLTSLQLQHL
FMILASPRYVDRVTEFLQQKLKQSQLLALKKELMV
QKQQEALEEQAALEPKLDLLLEKTKELQKLIEADI
SKRYSGRPVNLMGTSL- Isotype
- IgG
- Antibody clone number
- 1G4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references Suppression of the novel ER protein Maxer by mutant ataxin-1 in Bergman glia contributes to non-cell-autonomous toxicity.
Shiwaku H, Yoshimura N, Tamura T, Sone M, Ogishima S, Watase K, Tagawa K, Okazawa H
The EMBO journal 2010 Jul 21;29(14):2446-60
The EMBO journal 2010 Jul 21;29(14):2446-60
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- CDK5RAP3 monoclonal antibody (M01), clone 1G4 Western Blot analysis of CDK5RAP3 expression in A-431 ( Cat # L015V1 ).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged CDK5RAP3 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to CDK5RAP3 on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to CDK5RAP3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
