GTX48719

antibody from GeneTex

Targeting: PPP1R13B ASPP1, KIAA0771, p53BP2-like, p85

Western blot (Enhanced validation)

ELISA (Data presented on provider website)

Antibody Data

Product number

GTX48719

Provider

GeneTex

Proper citation

GeneTex Cat#GTX48719, RRID:AB_11166147

Product name

ASPP1 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Enhanced validation

Submitted by

GeneTex (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot using GeneTex's affinity purified anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells.

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

http://www.genetex.com

KD Reagent Prod no

GTX48719

KD Reagent Provider

Customized siRNA

Antibody Lot Number

39224

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Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Western blot using GeneTex's affinity purified anti-ASPP1 to detect over-expressed ASPP1 in MCF-7 cells (lane 2, arrowhead). Lane 1 is a non-transfected control. Lane 3 is MCF-7 cells over-expressing ASPP2. Cell extracts were electrophoresed and transferred to nitrocellulose. The membrane was probed with the primary antibody at a 1:1,000 dilution. The identity of the lower MW band at approximately 50kDa is unknown. Primary experimental data indicate that the unknown band intensifies in extracts from p53 siRNA knockdown cells. Personal Communication, H. Yang, Univ. Oklahoma, Oklahoma City, OK.

Validation comment

WB