AMAb90947

antibody from Atlas Antibodies

Targeting: MYH6

Antibody data

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Validation data

Product number: AMAb90947 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#AMAb90947, RRID:AB_2665728
Product name: Anti-MYH6
Antibody type: Monoclonal
Reactivity: Human
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: QVEEDKVNSLSKSKVKLEQQVDDLEGSLEQEKKVR MDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKL KKKEFDINQQNSKIEDEQVLALQLQKKLKENQARI EELEEELEAERTARAKVEKLRSDLSRELEEISERL EEA
Epitope: Binds to an epitope located within the peptide sequence LTQESIMDLENDKLQ as determined by overlapping synthetic peptides.
Isotype: IgG
Antibody clone number: CL2148
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

MYH6 protein structure - AMAb90947 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human heart muscle and liver tissues using AMAb90947 antibody. Corresponding MYH6 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic immunoreactivity in striated muscle fibers.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human liver shows absence of immunoreactivity (negative control).

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human heart muscle shows very strong cytoplasmic positivity in cardiomyocytes.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skeletal muscle shows strong cytoplasmic positivity in striated muscle fibers.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver shows no positivity in hepatocytes as expected.