H00006662-M01

antibody from Abnova Corporation

Targeting: SOX9 CMD1, CMPD1, SRA1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00006662-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00006662-M01, RRID:AB_535042

Product name

SOX9 monoclonal antibody (M01), clone 2A2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant SOX9.

Antigen sequence

EQLSPSHYSEQQQHSPQQIAYSPFNLPHYSPSYPP
ITRSQYDYTDHQNSSSYYSHAAGQGTGLYSTFTYM
NPAQRPMYTPIADTSGVPSIPQTHSPQHWEQPVYT
QLTRP


Isotype

IgG

Antibody clone number

2A2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Germ cells influence cord formation and leydig cell gene expression during mouse testis development.

Rios-Rojas C, Spiller C, Bowles J, Koopman P
Developmental dynamics : an official publication of the American Association of Anatomists 2016 Apr;245(4):433-44

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Primary cilia function regulates the length of the embryonic trunk axis and urogenital field in mice.

Wainwright EN, Svingen T, Ng ET, Wicking C, Koopman P
Developmental biology 2014 Nov 15;395(2):342-54

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody (M01), clone 2A2.Lane 1: SOX9 transfected lysate(63.639 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged SOX9 is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol