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- Antibody Data
- Antigen structure
- References [19]
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- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- AF2105 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Vimentin Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat Vimentin in Western blots.
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Autophagy promotes citrullination of VIM (vimentin) and its interaction with major histocompatibility complex class II in synovial fibroblasts.
Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/β‑catenin pathway.
Dexamethasone mediates pancreatic cancer progression by glucocorticoid receptor, TGFβ and JNK/AP-1.
Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium.
Morph-X-Select: Morphology-based tissue aptamer selection for ovarian cancer biomarker discovery.
TGF-β1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT).
Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols.
Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model.
Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes.
The mosaic of "seronegative" antiphospholipid syndrome.
Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein.
Angiotensin II contributes to renal fibrosis independently of Notch pathway activation.
In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
Fibroblast activation protein regulates tumor-associated fibroblasts and epithelial ovarian cancer cells.
Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems.
Vimentin/cardiolipin complex as a new antigenic target of the antiphospholipid syndrome.
Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension.
Morphogenesis and biological significance of spindle cell transformation in a spindle cell carcinoma.
Induction of epithelial-mesenchymal transition in primary airway epithelial cells from patients with asthma by transforming growth factor-beta1.
Sugawara E, Kato M, Kudo Y, Lee W, Hisada R, Fujieda Y, Oku K, Bohgaki T, Amengual O, Yasuda S, Onodera T, Hatakeyama S, Atsumi T
Autophagy 2020 May;16(5):946-955
Autophagy 2020 May;16(5):946-955
Zeylenone represses the progress of human prostate cancer by downregulating the Wnt/β‑catenin pathway.
Zeng S, Zhu B, Zeng J, Wu W, Jiang C
Molecular medicine reports 2018 Dec;18(6):5572-5578
Molecular medicine reports 2018 Dec;18(6):5572-5578
Dexamethasone mediates pancreatic cancer progression by glucocorticoid receptor, TGFβ and JNK/AP-1.
Liu L, Aleksandrowicz E, Schönsiegel F, Gröner D, Bauer N, Nwaeburu CC, Zhao Z, Gladkich J, Hoppe-Tichy T, Yefenof E, Hackert T, Strobel O, Herr I
Cell death & disease 2017 Oct 5;8(10):e3064
Cell death & disease 2017 Oct 5;8(10):e3064
Acute cigarette smoke exposure activates apoptotic and inflammatory programs but a second stimulus is required to induce epithelial to mesenchymal transition in COPD epithelium.
Murray LA, Dunmore R, Camelo A, Da Silva CA, Gustavsson MJ, Habiel DM, Hackett TL, Hogaboam CM, Sleeman MA, Knight DA
Respiratory research 2017 May 3;18(1):82
Respiratory research 2017 May 3;18(1):82
Morph-X-Select: Morphology-based tissue aptamer selection for ovarian cancer biomarker discovery.
Wang H, Li X, Volk DE, Lokesh GL, Elizondo-Riojas MA, Li L, Nick AM, Sood AK, Rosenblatt KP, Gorenstein DG
BioTechniques 2016;61(5):249-259
BioTechniques 2016;61(5):249-259
TGF-β1 Is Present at High Levels in Wound Fluid from Breast Cancer Patients Immediately Post-Surgery, and Is Not Increased by Intraoperative Radiation Therapy (IORT).
Scherer SD, Bauer J, Schmaus A, Neumaier C, Herskind C, Veldwijk MR, Wenz F, Sleeman JP
PloS one 2016;11(9):e0162221
PloS one 2016;11(9):e0162221
Differentiation of Human Induced-Pluripotent Stem Cells into Smooth-Muscle Cells: Two Novel Protocols.
Yang L, Geng Z, Nickel T, Johnson C, Gao L, Dutton J, Hou C, Zhang J
PloS one 2016;11(1):e0147155
PloS one 2016;11(1):e0147155
Targeting CXCR4 by a selective peptide antagonist modulates tumor microenvironment and microglia reactivity in a human glioblastoma model.
Mercurio L, Ajmone-Cat MA, Cecchetti S, Ricci A, Bozzuto G, Molinari A, Manni I, Pollo B, Scala S, Carpinelli G, Minghetti L
Journal of experimental & clinical cancer research : CR 2016 Mar 25;35:55
Journal of experimental & clinical cancer research : CR 2016 Mar 25;35:55
Establishment and characterization of an immortalized human hepatic stellate cell line for applications in co-culturing with immortalized human hepatocytes.
Pan X, Wang Y, Yu X, Li J, Zhou N, Du W, Zhang Y, Cao H, Zhu D, Chen Y, Li L
International journal of medical sciences 2015;12(3):248-55
International journal of medical sciences 2015;12(3):248-55
The mosaic of "seronegative" antiphospholipid syndrome.
Conti F, Capozzi A, Truglia S, Lococo E, Longo A, Misasi R, Alessandri C, Valesini G, Sorice M
Journal of immunology research 2014;2014:389601
Journal of immunology research 2014;2014:389601
Cellular vimentin regulates construction of dengue virus replication complexes through interaction with NS4A protein.
Teo CS, Chu JJ
Journal of virology 2014 Feb;88(4):1897-913
Journal of virology 2014 Feb;88(4):1897-913
Angiotensin II contributes to renal fibrosis independently of Notch pathway activation.
Lavoz C, Rodrigues-Diez R, Benito-Martin A, Rayego-Mateos S, Rodrigues-Diez RR, Alique M, Ortiz A, Mezzano S, Egido J, Ruiz-Ortega M
PloS one 2012;7(7):e40490
PloS one 2012;7(7):e40490
In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
Windus LC, Kiss DL, Glover T, Avery VM
Experimental cell research 2012 Nov 15;318(19):2507-19
Experimental cell research 2012 Nov 15;318(19):2507-19
Fibroblast activation protein regulates tumor-associated fibroblasts and epithelial ovarian cancer cells.
Lai D, Ma L, Wang F
International journal of oncology 2012 Aug;41(2):541-50
International journal of oncology 2012 Aug;41(2):541-50
Glycogene expression alterations associated with pancreatic cancer epithelial-mesenchymal transition in complementary model systems.
Maupin KA, Sinha A, Eugster E, Miller J, Ross J, Paulino V, Keshamouni VG, Tran N, Berens M, Webb C, Haab BB
PloS one 2010 Sep 27;5(9):e13002
PloS one 2010 Sep 27;5(9):e13002
Vimentin/cardiolipin complex as a new antigenic target of the antiphospholipid syndrome.
Ortona E, Capozzi A, Colasanti T, Conti F, Alessandri C, Longo A, Garofalo T, Margutti P, Misasi R, Khamashta MA, Hughes GR, Valesini G, Sorice M
Blood 2010 Oct 21;116(16):2960-7
Blood 2010 Oct 21;116(16):2960-7
Multipotent mesenchymal progenitor cells are present in endarterectomized tissues from patients with chronic thromboembolic pulmonary hypertension.
Firth AL, Yao W, Ogawa A, Madani MM, Lin GY, Yuan JX
American journal of physiology. Cell physiology 2010 May;298(5):C1217-25
American journal of physiology. Cell physiology 2010 May;298(5):C1217-25
Morphogenesis and biological significance of spindle cell transformation in a spindle cell carcinoma.
Kim EJ, Che ZM, Park YJ, Hwang YS, Kim KY, Jung DW, Jeon NK, Choi YW, Lee EJ, Kim J
Cancer letters 2009 Mar 8;275(1):61-71
Cancer letters 2009 Mar 8;275(1):61-71
Induction of epithelial-mesenchymal transition in primary airway epithelial cells from patients with asthma by transforming growth factor-beta1.
Hackett TL, Warner SM, Stefanowicz D, Shaheen F, Pechkovsky DV, Murray LA, Argentieri R, Kicic A, Stick SM, Bai TR, Knight DA
American journal of respiratory and critical care medicine 2009 Jul 15;180(2):122-33
American journal of respiratory and critical care medicine 2009 Jul 15;180(2):122-33
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Vimentin by Simple Western<SUP abp="263">TM. Detection of Human Vimentin by Simple Western<SUP abp="263">TM.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human Vimentin by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Vimentin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2105) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Vimentin at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat Vimentin by Western Blot.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Vimentin in Human Skin. Vimentin in Human Skin.
