AM00158PU-N

antibody from Acris Antibodies GmbH

Targeting: VIM

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AM00158PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AM00158PU-N, RRID:AB_1008515

Product name

anti Vimentin (incl. pos. control)

Antibody type

Monoclonal

Antigen

Synthetic peptide conjugated to hemocyanin. Epitope: N-terminus.

Reactivity

Human, Mouse, Canine

Host

Mouse

Isotype

IgG

Antibody clone number

11H6

Vial size

0.1 mg

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Detection of endogenous Vimentin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab Vim-11H6 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: HeLa; lane 2: HepG2; lane 3: HEK293; lane 4: SH-SY5Y; lane 5: MDCK; lane 6: PC12; lane 7: CMT 93; lane 8: Neuro 2A; lane 9: 3T3