AP31825PU-N

antibody from Acris Antibodies GmbH

Targeting: VIM

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

AP31825PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP31825PU-N, RRID:AB_11144390

Product name

anti Vimentin

Antibody type

Polyclonal

Antigen

Chickens were immunized with three synthetic peptide KLH conjugated corresponding to different regions of the Vimentin gene product, but are shared between the Human (NP_003371) and Mouse (NP_035831) sequences.Production: After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Reactivity

Human, Mouse

Host

Chicken/Avian

Vial size

0.3 mg

Concentration

0.3 mg/ml. The concentrations of each of the three antibodies was 0.1 mg/ml (based on Bradford assay readings using BSA as a standard).

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

In the Left panel (a tissue section through an e18 Mouse cerebellum), Vimentin (brown staining) can be seen in immature astrocytes of the white matter, as well as in vascular endothelial cells. In the Right panel (cultured dissociated cells from an e13 Mouse brain), vimentin (green staining) can be seen forming the cytoskeleton of immature astrocyte progenitor cells. DAPI (blue staining) allows visualization of nuclei.