MA5-14435

antibody from Invitrogen Antibodies

Targeting: VIM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-14435

Provider

Invitrogen Antibodies

Product name

Vimentin Antibody Cocktail

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA5-14435 targets Vimentin in IHC (P) applications and shows reactivity with Human samples.

Antibody clone number

Vimentin-SP20, gp100-HMB45

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts of HeLa (Lane 1), MDA-MB-231 (Lane 2), Daudi (Lane 3), Raji (Lane 4) and Ramos (Lane 5). The blot was probed with Anti-Vimentin Antibody Cocktail (Product # MA5-14435, 100 ul) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution), Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution) and Rabbit anti-Goat IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27014, 0.25 µg/ml, 1:4000 dilution) as the host species was mixed category. A 55 kDa band corresponding to Vimentin was observed across the cell lines tested except in the cell lines of the B-lymphocyte class.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Formalin-fixed paraffin-embedded human melanoma stained with the MultiVision anti-Mouse/ HRP + anti-Rabbit/AP. Note: Cytoplasmic staining of Vimentin (in blue) and gp100 (in red).