PA5-11570

antibody from Invitrogen Antibodies

Targeting: ACTG2 ACTA3, ACTL3, ACTSG

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-11570

Provider

Invitrogen Antibodies

Product name

Actin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, chicken, canine, equine, hamster, porcine, non-human primate, rat, rabbit and Xenopus, yeast and zebrafish based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Vial size

400 µL

Concentration

1.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Bisphenol A, Bisphenol F, and Bisphenol S: The Bad and the Ugly. Where Is the Good?

Fouyet S, Olivier E, Leproux P, Dutot M, Rat P
Life (Basel, Switzerland) 2021 Apr 3;11(4)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using an Actin polyclonal antibody (Product # PA5-11570) in mouse testis tissue lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A549 (Lane 2), COS-7 (Lane 3), MDCK (Lane 4), NIH/3T3 (Lane 5) tissue extracts of and Rat Skeletal Muscle (Lane 6). The blot was probed with Actin Polyclonal Antibody (Product # PA5-11570, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to Actin was observed across the cell lines and tissues tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on whole cell extracts (30 µg lysate) of A-431 (Lane 1), A549 (Lane 2), COS-7 (Lane 3), MDCK (Lane 4), NIH/3T3 (Lane 5) tissue extracts of and Rat Skeletal Muscle (Lane 6). The blot was probed with Actin Polyclonal Antibody (Product # PA5-11570, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 42 kDa band corresponding to Actin was observed across the cell lines and tissues tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HeLa cells using an Actin polyclonal antibody (Product # PA5-11570) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Actin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Actin Polyclonal Antibody (Product # PA5-11570) at 1:50 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoskeletal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lung carcinoma tissue using an Actin polyclonal antibody (Product # PA5-11570), followed by HRP-conjugated secondary antibody and DAB staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 ( a - c ) P2X7 receptor activation was evaluated after ( a ) BPA, ( b ) BPF, and ( c ) BPS incubation for 72 h in JEG-Tox cells. Data correspond to the mean +-SEM of four independent experiments. The significance thresholds were **** p < 0.0001, *** p < 0.001, and ** p < 0.01 compared to the control. ( d , e ) P2X7 receptor expression was assessed after BPA, BPF, and BPS incubation for 72 h in JEG-Tox cells ( d ) using the Western blot technique, and ( e ) quantitative analysis was performed using ImageJ software. beta-actin was used as a control.