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LearnR32059
antibody from NSJ Bioreagents
Targeting: AGTR1
AG2S, AGTR1A, AGTR1B, AT1, AT1B, AT2R1, AT2R1A, AT2R1B, HAT1R
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- R32059 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Angiotensin II Type 1 Receptor Antibody (AGTR1, AT1R)
- Antibody type
- Polyclonal
- Antigen
- Amino acids MILNSSTEDGIKRIQDDCPKAGRHNYIF of human AGTR1 were used as the immunogen for the AT1R antibody.
- Description
- Antigen affinity
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the AT1R antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) HeLa and 2) HEPA cell lysate with AT1R antibody. Observed molecular weight ~41-50 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast cancer tissue with AT1R antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
