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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- TA319476 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-DNMT3L antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-DNMT3L antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- DNMT3L
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.11 mg/mL
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Supportive validation
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- OriGene (provider)
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- Experimental details
- Rabbit anti-DNMT3L was used at a 1:1,500 dilution to detect human DNMT3L by WB after IP using the same antibody.? Transfection of U2OS cells (10cm dish) was accomplished using 3 ug of GAL4-DNMT3L. Protein extraction using IPH at 150mM. For WB the antibody was used at 0.4ug/ml in TBS milk 2%, BSA 0.5%. Detection occurred using ECL.?
- Validation comment
- WB
