Explore
Validate
Learn
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-9534-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AIRE Monoclonal Antibody (TM-724), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This TM-724 monoclonal antibody reacts with human Autoimmune Regulator (AIRE). This 58 kDa transcription factor is highly expressed within the perinuclear region of mature medullary thymic epithelial cells (mTECs). AIRE mRNA in the periphery has been observed in the lymph node, fetal liver, and intestines. Furthermore expression has been observed in dendritic cells, epithelial cells and PBMCs. Studies demonstrate that AIRE is involved in thymocyte development, in particular the regulation of tissue-specific antigen expression during negative selection. Other biological functions for AIRE include chemokine expression and antigen presentation. As a result of alternative splicing, three isoforms have been reported. The conditions autoimmune-polyendocrinopathy-candiasis-ectodermal-dystrophy (APECED) and autoimmune polyglandular syndrome type 1 (APS-1) arise from mutations in the human AIRE gene.
- Antibody clone number
- TM-724
- Concentration
- 0.5 mg/mL
Submitted references Expression and prognostic significance of the autoimmune regulator gene in breast cancer cells.
The Ubiquitin-like Modifier FAT10 Is Selectively Expressed in Medullary Thymic Epithelial Cells and Modifies T Cell Selection.
Editorial overview.
Human peripheral lymphoid tissues contain autoimmune regulator-expressing dendritic cells.
Expression of AIRE in thymocytes and peripheral lymphocytes.
Bianchi F, Sommariva M, De Cecco L, Triulzi T, Romero-Cordoba S, Tagliabue E, Sfondrini L, Balsari A
Cell cycle (Georgetown, Tex.) 2016 Dec;15(23):3220-3229
Cell cycle (Georgetown, Tex.) 2016 Dec;15(23):3220-3229
The Ubiquitin-like Modifier FAT10 Is Selectively Expressed in Medullary Thymic Epithelial Cells and Modifies T Cell Selection.
Buerger S, Herrmann VL, Mundt S, Trautwein N, Groettrup M, Basler M
Journal of immunology (Baltimore, Md. : 1950) 2015 Nov 1;195(9):4106-16
Journal of immunology (Baltimore, Md. : 1950) 2015 Nov 1;195(9):4106-16
Editorial overview.
Glimcher LH, Martinon F, Modlin RL
Current opinion in immunology 2011 Feb;23(1):1-2
Current opinion in immunology 2011 Feb;23(1):1-2
Human peripheral lymphoid tissues contain autoimmune regulator-expressing dendritic cells.
Poliani PL, Kisand K, Marrella V, Ravanini M, Notarangelo LD, Villa A, Peterson P, Facchetti F
The American journal of pathology 2010 Mar;176(3):1104-12
The American journal of pathology 2010 Mar;176(3):1104-12
Expression of AIRE in thymocytes and peripheral lymphocytes.
Suzuki E, Kobayashi Y, Kawano O, Endo K, Haneda H, Yukiue H, Sasaki H, Yano M, Maeda M, Fujii Y
Autoimmunity 2008 Mar;41(2):133-9
Autoimmunity 2008 Mar;41(2):133-9
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human thymus stained with 5 µg/mL of Rat IgG2a Isotype Control (left) or 5 µg/mL Anti-Human AIRE Purified (right) followed by Anti-Rat IgG Biotin and DAB visualization.Nuclei are counterstained with hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
