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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB792-100 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal IGF-II/IGF2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IGF-II/IGF2 in ELISAs and Western blots. In ELISAs, this antibody shows less than 6% cross-reactivity with recombinant human (rh) IGF-I R and no cross-reactivity with rhIGF-I and rmIGF-I is observed. In Western blots, 100% cross-reactivity with rhIGF-II and no cross reactivity with rmIGF-I is observed.
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of Recombinant Human and Mouse IGF-II/IGF2 by Western Blot. Western blot shows 50 ng of Recombinant Mouse IGF-I/IGF1 (Catalog # 791-MG), Recombinant Human IGF-II/IGF2 (Catalog # 292-G2) and Recombinant Mouse IGF-I/IGF1 (Catalog # 791-MG). PVDF Membrane was probed with 1 µg/mL of Rat Anti-Mouse IGF-II/IGF2 Monoclonal Antibody (Catalog # MAB792) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for IGF-II/IGF2 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
