R1495P

antibody from Acris Antibodies GmbH

Targeting: RAD52

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

R1495P

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#R1495P, RRID:AB_1006843

Product name

anti RAD52

Antibody type

Polyclonal

Antigen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 360-375 of the Human Rad 52 protein.

Reactivity

Human

Host

Rabbit

Vial size

0.1 mg

Concentration

1.0 mg/ml (by UV absorbance at 280 nm)

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Western blot analysis is shown using Affinity Purified anti-Human RAD52 antibody to detect Human RAD52 present in a HeLa nuclear extract (panel B). ~30 µg of lysate was loaded per lane for 4-20% gradient SDS-PAGE.Comparison to a molecular weight marker (not shown) indicates a band of ~63.0 kDa is detected. Peptide competition (panel A) blocks the specific staining of this band. The blot was incubated with a 1:1000 dilution of the antibody at roomtemperature for 2 h followed by detection using IRDye TM800 labeled Goat-a-Rabbit IgG [H&L] diluted 1:5,000 for 45 min. IRDye TM800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed byLI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.