Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP1-47518PE - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Calreticulin Antibody
- Antibody type
- Monoclonal
- Description
- Ammonium sulfate precipitation.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): Calreticulin Antibody (1G6A7) [Phycoerythrin] [NBP1-47518PE] - Using the PE direct conjugate, an intracellular stain was performed on HeLa cells with Calreticulin (1G6A7) antibody NBP1-47518PE(blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.