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LearnPA5-51700
antibody from Invitrogen Antibodies
Targeting: BIRC3
API2, c-IAP2, cIAP2, hiap-1, MALT2, MIHC, RNF49
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [4]
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- Product number
- PA5-51700 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- cIAP2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QHAKWFPRCE YLIRIKGQEF IRQVQASYPH LLEQLLSTSD SPGDENAESS IIHFEPGEDH SEDAIMMNTP VINAAVEMGF SRSLVKQTVQ RKILATGENY RLVNDLVLDL LNAEDEIREE ERERATEE
- Concentration
- 0.15 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of cIAP2 in control (vector only transfected HEK293T lysate) and BIRC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of cIAP2 in human cell line Daudi using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of cIAP2 in mouse cell line NIH-3T3 and rat cell line NBT-II using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of cIAP2 in control (vector only transfected HEK293T lysate) and BIRC3 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-cIAP2 Polyclonal Antibody (Product # PA5-51700) and 68, 72 kDa bands corresponding to cIAP2 were observed in Raji, Daudi and Mouse Thymus but not in BeWo, HEL 92.1.7, IMR-32, SH-SY5Y and Mouse Brain. Nuclear enriched extracts (30 µg lysate) of Raji (Lane 1), Daudi (Lane 2), BeWo (Lane 3), HEL 92.1.7 (Lane 4), IMR-32 (Lane 5), SH-SY5Y (Lane 6), Mouse Thymus (Lane 7), Mouse Brain (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of cIAP2 in mouse piriform cortex shows cytoplasmic positivity in neurons. Samples were probed using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of cIAP2 in human cell line U-251 MG shows positivity in cytoplasm & nucleus but excluded from the nucleoli. Samples were probed using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of cIAP2 in mouse cerebellum tissue shows strong immunoreactivity in Purkinje cells. Samples were probed using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of cIAP2 in mouse olfactory bulb shows cytoplasmic staining in mitral and external plexiform cell layers. Samples were probed using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of cIAP2 in mouse thalamus shows neuronal staining in paraventricular nucleus. Samples were probed using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of cIAP2 in mouse hippocampus shows cytoplasmic positivity in a subset of neurons. Samples were probed using a cIAP2 Polyclonal Antibody (Product # PA5-51700).
