TA501054

antibody from OriGene

Targeting: TPMT

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA501054

Provider

OriGene

Proper citation

OriGene Cat#TA501054, RRID:AB_11140731

Product name

Anti-TPMT mouse monoclonal antibody, clone OTI4C1 (formerly 4C1)

Antibody type

Monoclonal

Description

Anti-TPMT mouse monoclonal antibody, clone OTI4C1 (formerly 4C1)

Reactivity

Canine

Host

Mouse

Conjugate

Unconjugated

Epitope

TPMT

Isotype

IgG

Antibody clone number

OTI4C1

Vial size

100 µl

Concentration

0.88 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TPMT (RC203309, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TPMT.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

WB analysis of extracts (35ug) from 9 different cell lines by using anti-TPMT monoclonal antibody.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (TA501062, Dilution 1:50; heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501054, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-TPMT mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA501054, Dilution 1:50)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either pCMV6-ENTRY TPMT(RC203309)(Red) or empty vector control plasmid(Blue) were immunostained with anti-TPMT mouse monoclonal(TA501054), and then analyzed by flow cytometry.

Validation comment

FC