MA1-25333

antibody from Invitrogen Antibodies

Targeting: E2F3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA1-25333

Provider

Invitrogen Antibodies

Product name

E2F3 Monoclonal Antibody (PG30)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

PG30

Vial size

100 µL

Concentration

2.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-E2F3 Mouse Monoclonal Antibody (Product # MA1-25333) and a 52 kDa bands corresponding to E2F3 was observed across cell line tested and increased upon treatment with Doxorubicin and Etoposide. Whole cell extracts (30 µg lysate) of U2OS (Lane 1), U2OS treated with Doxorubicin (300ng/ml for 12 Hours) (Lane 2), U2OS treated with Etoposide (6µg/ml for 12 Hours) (Lane 3) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).