orb148096

antibody from Biorbyt

Targeting: DNMT1 CXXC9, DNMT, MCMT

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

orb148096

Provider

Biorbyt

Product name

DNMT1 antibody (PE/ATTO-594)

Antibody type

Monoclonal

Description

Mouse monoclonal to DNMT1 (PE/ATTO-594). Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F-binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells (1-6)..

Reactivity

Human, Zebrafish

Host

Mouse

Conjugate

Red dye

Isotype

IgG

Antibody clone number

4G11-C7

Vial size

100 μg

Concentration

1 mg/ml

Storage

Store at 2-8°C and -20°C for one year. (Shipped on ice packs)