Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA3-16556 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-DNMT1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- This antibody does not neutralize the enzymatic activity of its corresponding methyltransferase. Suggested positive control: T24 cell lysates.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- Conc. Not Determined
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis using Product # PA3-16556 to detect DNMT1 levels in T24 degrees Cells. Lane 1: 50 ugs of total protein lysate from untreated T24 degrees Cells. Lane 2: 50 ugs of total protein lysate treated with 0.5 uM 5-Aza-Deoxycytidine for 3 days.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of DNMT1 in nuclei of Hela cells using a polyclonal antibody (Product # PA3-16556).