PA5-22172

antibody from Invitrogen Antibodies

Targeting: PFKM PFK-1, PFKX, PPP1R122

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-22172

Provider

Invitrogen Antibodies

Product name

PFKM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, mouse brain, rat brain.

Concentration

0.92 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PFK-1 using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PFK-1 polyclonal antibody (Product # PA5-22172) at a dilution of 1:2000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PFK-1 using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PFK-1 polyclonal antibody (Product # PA5-22172) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PFKM was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PFKM Polyclonal Antibody (Product # PA5-22172) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PFKM Polyclonal Antibody (Product # PA5-22172). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM Polyclonal Antibody (Product # PA5-22172) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using PFKM Polyclonal Antibody (Product # PA5-22172). Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PFKM Polyclonal Antibody (Product # PA5-22172) diluted at 1:2,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.