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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PA5-29336 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PFKM Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, mouse brain, rat brain.
- Concentration
- 0.63 mg/mL
Submitted references The deubiquitinase JOSD2 is a positive regulator of glucose metabolism.
Krassikova L, Zhang B, Nagarajan D, Queiroz AL, Kacal M, Samakidis E, Vakifahmetoglu-Norberg H, Norberg E
Cell death and differentiation 2021 Mar;28(3):1091-1109
Cell death and differentiation 2021 Mar;28(3):1091-1109
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PFK (muscle) using 50 µg of mouse brain lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a PFK (muscle) polyclonal antibody (Product # PA5-29336) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of C2C12 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), NIH/3T3 (Lane 4) and HEK 293 (Lane 5). The blot was probed with Anti-PFKM Polyclonal Antibody (Product # PA5-29336, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 85 kDa band corresponding to PFKM was observed across the cell lines tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PFKM was performed by separating 50 µg of Various tissue extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PFKM Polyclonal Antibody (Product # PA5-29336) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using PFKM Polyclonal Antibody (Product # PA5-29336). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: H1299. 7.5% SDS PAGE. PFKM Polyclonal Antibody (Product # PA5-29336) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of PFKM was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a PFKM Polyclonal Antibody (Product # PA5-29336) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PFK (muscle) in methanol-fixed A431 cells using a PFK (muscle) polyclonal antibody (Product # PA5-29336) at a 1:200 dilution.
