MA5-17235

antibody from Invitrogen Antibodies

Targeting: UCHL1 PARK5, PGP9.5, Uch-L1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

MA5-17235

Provider

Invitrogen Antibodies

Product name

Anti-PGP9.5 Monoclonal Antibody (AF3F8)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is SK-N-MC cells.

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

AF3F8

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of UCHL1 using anti-UCHL1 monoclonal antibody (Product # MA5-17235) on (Lane 1) U87mg Cell lysate, (Lane 2) SH-DY5Y Cell lysate, (Lane 3) SK-N-MC Cell lysate, (Lane 4) SK-N-SH Cell lysate, (Lane 5) IMR32 Cell lysate, (Lane 6) PC12 Cell lysate and (Lane 6) Mouse Brain.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-PGP9.5 Monoclonal Antibody, (Product # MA5-17235) and a ~25 kDa band corresponding to PGP9.5 was observed across the panel tested except SW480, HT-29, HeLa and HepG2 which are reported to be negative. Whole cell extracts (30 µg lysate) of SH-SY5Y (Lane 1), U-87 MG (Lane 2), IMR-32 (Lane 3), SK-N-SH (Lane 4), A549 (Lane 5), SW480 (Lane 6), HT-29 (Lane 7), HeLa (Lane 8), HepG2 (Lane 9) and Neuro-2a (Lane 10) were electrophoresed using Novex® NuPAGE® 12% % Bis-Tris gel (Product # NP0341BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5µg/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005)..

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PGP9.5/UCHL1 was performed by loading 20 µg of SH-SY5Y wild type (Lane 1), SH-SY5Y Cas9 control (Lane 2), SH-SY5Y PGP9.5/UCHL1 knockout (Lane 3) whole cell extracts. The blot was probed with Anti-PGP9.5/ UCHL1 Monoclonal Antibody (Product # MA5-17235) (0.5 µg/mL) and Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177) (1:4000 dilution). Loss of signal upon CRISPR mediated knockout (KO) confirms that antibody is specific to PGP9.5/UCHL1.