PAB12509

antibody from Abnova Corporation

Targeting: UCHL1 PARK5, PGP9.5, Uch-L1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB12509

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB12509, RRID:AB_10678512

Product name

UCHL1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against full length recombinant UCHL1.

Storage

Store at 4°C for short term. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

The UCH-L1 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility.

Liu Y, Fallon L, Lashuel HA, Liu Z, Lansbury PT Jr
Cell 2002 Oct 18;111(2):209-18

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Loss of Uch-L1 and Uch-L3 leads to neurodegeneration, posterior paralysis and dysphagia.

Kurihara LJ, Kikuchi T, Wada K, Tilghman SM
Human molecular genetics 2001 Sep 1;10(18):1963-70

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The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.

Wilkinson KD, Lee KM, Deshpande S, Duerksen-Hughes P, Boss JM, Pohl J
Science (New York, N.Y.) 1989 Nov 3;246(4930):670-3

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of whole bovine brain extract stained with UCHL1 polyclonal antibody (Cat # PAB12509) showing a strong and clean band at about 24kDa.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Shows rat mixed neuron/glial cultures stained with UCHL1 polyclonal antibody (Cat # PAB12509) (green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also.

Validation comment

Immunofluorescence