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- Antibody Data
- Antigen structure
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- Product number
- AF6007 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse/Rat UCH-L1/PGP9.5 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human, mouse, and rat UCH-L1/PGP9.5 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human UCH-L3 is observed.
- Reactivity
- Human, Mouse, Rat
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
P09936- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human, Mouse, and Rat UCH-L1/PGP9.5 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, Neuro-2A mouse neuroblastoma cell line, PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/ Rat UCH-L1/PGP9.5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6007) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for UCH-L1/PGP9.5 at approximately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse UCH-L1 by Simple WesternTM. Simple Western lane view shows lysates of A172 human glioblastoma cell line and Neuro-2A mouse neuroblastoma cell line, loaded at 0.2 mg/mL. A specific band was detected for UCH-L1/ PGP9.5 at approximately 30 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human/Mouse/Rat UCH-L1/PGP9.5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6007) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
