AB-250-NA

antibody from Novus Biologicals

Targeting: LIF CDF, DIA, HILDA

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-250-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal LIF Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects human LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse LIF is observed. Neutralizes the biological activity of recombinant human LIF and will also neutralize the biological activity of recombinant mouse LIF at a 50-fold higher IgG concentration.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Leukemia inhibitory factor functions in parallel with interleukin-6 to promote ovarian cancer growth.

McLean K, Tan L, Bolland DE, Coffman LG, Peterson LF, Talpaz M, Neamati N, Buckanovich RJ
Oncogene 2019 Feb;38(9):1576-1584

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF (Catalog # 7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by 0.5 ng/mL Recom-binant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Polyclonal Antibody (Catalog # AB-250-NA). The ND₅₀ is typically 0.1-0.4 µg/mL.