PA5-40966

antibody from Invitrogen Antibodies

Targeting: TRIM9 RNF91, SPRING

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-40966

Provider

Invitrogen Antibodies

Product name

TRIM9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: DGLHFNSTYN ARVKAFNKTG VSPYSKTLVL QTSEGKALQQ YPSERELRGI

Concentration

0.5 mg/mL

References

Submitted references

Knockdown of TRIM9 attenuates irinotecan‑induced intestinal mucositis in IEC‑6 cells by regulating DUSP6 expression via the P38 pathway.

Zhao W, Wang Q
Molecular medicine reports 2021 Dec;24(6)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human 721_B cell lysate using an anti-TRIM9 polyclonal antibody (Product # PA5-40966).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 2. Interference and overexpression efficiency of TRIM9.IEC-6 cells were treated with TRIM9 overexpression or interference lentiviruses. (A) Representative bar graphs showing that TRIM9 mRNA expression was significantly increased in the oeTRIM9 group and reduced in the shTRIM9-1, -2, -3 groups. (B) TRIM9 protein expression was significantly increased in the oeTRIM9 group and reduced in the shTRIM9-1, -2, -3 groups. ***P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Knockdown of TRIM9 partly inhibits the irinotecan-induced inhibition of cell proliferation, intestinal barrier function impairment and inflammatory factor expression levels. IEC-6 cells were treated with irinotecan combined with TRIM9 interference. (A) Cell proliferation at 0, 12, 24 and 48 h was detected using a Cell Counting Kit-8 assay. (B) TEER was measured in each group. (C) The fluorescence intensity of FITC was tested in each group. (D) Cell apoptosis was detected with a flow cytometer. (E) The levels of IL-1beta and TNF-alpha expression were detected with an ELISA. (F) The protein expression levels of TRIM9, ZO-1, Claudin-4 and p-P38/P38 were detected via western blotting. **P

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 5. TRIM9 directly interacts with DUSP6 in IEC-6 cells. (A) The protein expression levels of TRIM9, DUSP1, DUSP4, DUSP5, DUSP6 and DUSP10 in IEC-6 cells transfected with oeTRIM9 was detected. **P