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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [4]
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- Western blot [2]
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- Product number
- AF789 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SMAC/Diablo Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SMAC/Diablo in Western blots. Also detects mouse and rat SMAC/Diablo when the salt concentration of blotting buffer is reduced to 0.15 M NaCl, and 1 µg/mL anti-SMAC/Diablo concentration is used.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Pro-Apoptotic Activity of New Honokiol/Triphenylmethane Analogues in B-Cell Lymphoid Malignancies.
Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling.
Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia.
Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3.
Mędra A, Witkowska M, Majchrzak A, Cebula-Obrzut B, Bonner MY, Robak T, Arbiser JL, Smolewski P
Molecules (Basel, Switzerland) 2016 Jul 30;21(8)
Molecules (Basel, Switzerland) 2016 Jul 30;21(8)
Proteasome inhibition can impair caspase-8 activation upon submaximal stimulation of apoptotic tumor necrosis factor-related apoptosis inducing ligand (TRAIL) signaling.
Laussmann MA, Passante E, Hellwig CT, Tomiczek B, Flanagan L, Prehn JH, Huber HJ, Rehm M
The Journal of biological chemistry 2012 Apr 27;287(18):14402-11
The Journal of biological chemistry 2012 Apr 27;287(18):14402-11
Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia.
Wang H, Jiang JY, Zhu C, Peng C, Tsang BK
Molecular endocrinology (Baltimore, Md.) 2006 Oct;20(10):2469-82
Molecular endocrinology (Baltimore, Md.) 2006 Oct;20(10):2469-82
Inhibition of lymphotoxin-beta receptor-mediated cell death by survivin-DeltaEx3.
You RI, Chen MC, Wang HW, Chou YC, Lin CH, Hsieh SL
Cancer research 2006 Mar 15;66(6):3051-61
Cancer research 2006 Mar 15;66(6):3051-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SMAC/Diablo by Western Blot. Western blot shows lysates of SH-SY5Y human neuroblastoma cell line, CHP-100 human neuroblastoma cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human SMAC/Diablo (Catalog # 789-SM) (0.5 ng/lane) was included. A specific band for SMAC/Diablo was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SMAC/Diablo by Simple WesternTM. Simple Western lane view shows lysates of SH-SY5Y human neuroblastoma cell line and Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for SMAC/Diablo at approximately 29 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human SMAC/Diablo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF789). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
