PAB29316

antibody from Abnova Corporation

Targeting: DIABLO DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB29316

Provider

Abnova Corporation

Product name

DIABLO polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant human DIABLO.

Antigen sequence

AVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGAR
AEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTG
ADQASITARNHIQLVKLQVEEVHQLSRKAETKLAE
AQIEELRQKTQEEG


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells) with DIABLO polyclonal antibody (Cat# PAB29316) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with DIABLO polyclonal antibody (Cat# PAB29316) under 1-4 ug/mL working concentration shows positivity in mitochondria.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human testis with DIABLO polyclonal antibody (Cat# PAB29316) shows strong cytoplasmic positivity in cells of seminiferus ducts at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)