PA5-28495
antibody from Invitrogen Antibodies
Targeting: DIABLO
DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28495 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DIABLO Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, mouse testis.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DIABLO was performed by separating 50 µg of mouse tissue extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a DIABLO Polyclonal Antibody (Product # PA5-28495) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using DIABLO Polyclonal Antibody (Product # PA5-28495). Sample (30 µg of whole cell lysate). Lane A: A431. 12% SDS PAGE. DIABLO Polyclonal Antibody (Product # PA5-28495) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-DIABLO Polyclonal Antibody(Product # PA5-28495) and a 21kDa band corresponding to DIABLO was observed across the cell lines and tissues tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF7 (Lane 2), Hep G2 (Lane 3), Mouse Testis (Lane 4), Mouse Skeletal Muscle (Lane 5) Mouse Brain (Lane 6) and Mouse Heart (Lane 8), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # LC2001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Diablo (Product # PA5-28495) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.