- UM800073 - OriGene RTRAF antibody

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Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-C14orf166 monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549. 5: Jurkat, 6: MDCK, 7: COS7, 8: PC-12, 9: MCF7).
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunofluorescent staining of HeLa cells using anti-C14orf166 mouse monoclonal antibody (UM800073, green, 1:100). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800073)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800073)(1:200)

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800073)(1:200)

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800073)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800073)(1:200)

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110 °C for 10 min, UM800073)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human colon cancer using anti-C14ORF166 clone UMAB181 mouse monoclonal antibody at 1:600 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800073 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells have strong nuclear and weak cytoplasmic staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded human thyroid using anti-C14ORF166 clone UMAB181 mouse monoclonal antibody at 1:600 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800073 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows strong nuclear and intermediate levels of cytoplasmic staining on the thyroid.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human kidney using anti-C14ORF166 clone UMAB181 mouse monoclonal antibody at 1:600 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800073 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells have strong nuclear and intermediate levels of cytoplasmic staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: OriGene overexpression protein microarray chip was immunostained with UltraMAB anti-C14orf166 mouse monoclonal antibody (UM800073). The positive reactive proteins are highlighted with two red arrows in the enlarged subarray. All the positive controls spotted in this subarray are also labeled for clarification.(1:100)
Validation comment: 10K-CHIP

UM800073