14-9176-80

antibody from Invitrogen Antibodies

Targeting: BIRC5 API4, EPR-1, survivin

Antibody data

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Validation data

Product number: 14-9176-80 - Provider product page
Provider: Invitrogen Antibodies
Product name: Survivin Monoclonal Antibody (STLALYV), eBioscience™
Antibody type: Monoclonal
Antigen: Other
Description: Description: This STLALYV monoclonal antibody recognizes human survivin (also known as Birc5). Survivin is a member of the inhibitor of apoptosis (IAP) family that also includes XIAP, c-IAP1, c-IAP2, and NAIP. Survivin functions to inhibit caspase activation, thereby leading to negative regulation of apoptosis. Survivin also plays a critical role in mitosis and cytokinesis where it is expressed only during G2/M and localizes to the mitotic spindle and is found at the cleavage furrow/midplate. Thus, survivin is highly expressed in tumors and in fetal tissue, but is absent in terminally-differentiated cells and non-proliferating cells. Deletion of survivin in developing thymocytes as well as in mature T cells showed that survivin is critical for cell division in lymphoid cells but not for inhibition of survival; the same is true for B lymphocytes.
Antibody clone number: STLALYV
Concentration: 0.5 mg/mL

BIRC5 protein structure - 14-9176-80 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry of fixed and permeabilized HeLa cells using 5 µg/mL Anti-Human Survivin Purified, followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry of fixed and permeabilized HeLa cells using 5 µg/mL Anti-Human Survivin Purified, followed by F (ab')2 Anti-Mouse IgG eFluor® 570.Nuclei are stained with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Survivin was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Survivin Monoclonal Antibody (STLALYV), eBioscience™ (Product # 14-9176-82) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing Nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.