PA5-91872

antibody from Invitrogen Antibodies

Targeting: PTGER2 EP2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-91872

Provider

Invitrogen Antibodies

Product name

PTGER2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Positive Samples: K-562, SW480, 22Rv1, OVCAR3; Cellular Location: Cell membrane, Multi-pass membrane protein

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using PTGER2 Polyclonal antibody (Product # PA5-91872) at 1:200 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 90s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of PTGER2 in extracts of various cell lines using PTGER2 Polyclonal Antibody (Product # PA5-91872) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of PTGER2 was performed in HeLa cells using PTGER2 Polyclonal Antibody (Product # PA5-91872).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PTGER2 in paraffin-embedded mouse lung using PTGER2 Polyclonal Antibody (Product # PA5-91872) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PTGER2 in paraffin-embedded mouse lung using PTGER2 Polyclonal Antibody (Product # PA5-91872) at a dilution of 1:200.