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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP15745PU-N - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal anti-Prohibitin / PHB antibody, Purified
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal anti-Prohibitin / PHB antibody, Purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 1ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western Blot analysis using Prohibitin / PHB?Antibody Cat.-No AP15745PU-S at 2 ug/ml in 1 mg/ml lysate, HeLa 10 ug (Lane 1+2), Raw 20ug (Lane 3+4) lysate, blocking buffer (Blotto; 5% dry milk powder in TBST). R1634HRP anti-Rabbit IgG (H+L)-HRP secondary antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Prohibitin / PHB?Antibody Cat.-No AP15745PU-S on Raw 204.4 at 5 ug/ml (Left), incubated with F(ab??)2 Goat anti rabbit IgG (H+L)-FITC conjugated secondary antibody ((R1410F, 1/100). Cells were fixed in 1% PFA, permeabilized in 0.25%Triton X 100 in PBS, blocked in 1% BSA in PBS.
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Human Heart stained with Anti-Prohibitin
- Validation comment
- IHC
