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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10296 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB10296, RRID:AB_11188566
- Product name
- PHB monoclonal antibody, clone 5H7
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against recombinant PHB.
- Isotype
- IgG
- Antibody clone number
- 5H7
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) against A-431 (1), MCF-7 (2), Jurkat (3), HeLa (4), HepG2 (5), A-549 (6), NIH/3T3 (7), COS-7 (8) and PC-12 (9) cell lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) against recombinant human PHB protein.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of NIH/3T3 cells using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue (A), liver cancer tissue (B), lung cancer tissue (C) and stomach cancer tissue (D) using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) with DAB staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of MCF-7 cells using PHB monoclonal antobody, clone 5H7 (Cat # MAB10296) (blue) and negative control (red).
- Validation comment
- Flow Cytometry
