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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NBP2-19713 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal PBX1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PBX1 Antibody [NBP2-19713] - Sample (30 ug of whole cell lysate) A: A431 10% SDS PAGE gel, diluted at 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PBX1 Antibody [NBP2-19713] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with PBX1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PBX1 Antibody [NBP2-19713] - 293T whole cell lysate/extract A. Control with 2 ug of preimmune rabbit IgG B. Immunoprecipitation of PBX1 protein by 2 ug of PBX1 antibody 12 % SDS-PAGE The immunoprecipitated PBX1 protein was detected by PBX1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
